Lee S H, Aggarwal B B, Rinderknecht E, Assisi F, Chiu H
J Immunol. 1984 Sep;133(3):1083-6.
Highly purified preparations of rHuIFN-gamma and HLT synergistically inhibit the in vitro proliferation of WI38VA13 cells, a SV40 virus-transformed derivative of the human cell line W138. The growth of nontumorigenic WI38 cells, established from normal diploid embryonic lung tissue, was not inhibited under identical incubation conditions; rMuIFN-gamma and HLT have the same in vitro effects on the murine melanoma B16. Thus, the combination of rMuIFN-gamma and HLT synergistically inhibits the growth of B16 cells, although incubation with either lymphokine alone has no significant anti-proliferative effect. Flow cytometric analysis of the cell cycle of asynchronous cells indicates that when B16 melanoma cells are treated with rMuIFN-gamma and HLT in combination, more cells accumulate in the G0/G1 phase than when the cells are treated with rMuIFN-gamma alone. It appears that populations of rapidly proliferating tumor cells, which normally contain a large percentage in the S+G2+M phases of the cell cycle, become arrested in the quiescent G0/G1 phase by the combination of IFN-gamma and HLT, resulting in the inhibition of cell growth.
高纯度的重组人γ干扰素（rHuIFN-γ）制剂与热灭活淋巴细胞（HLT）协同抑制WI38VA13细胞的体外增殖，WI38VA13细胞是人类细胞系W138的SV40病毒转化衍生物。从正常二倍体胚胎肺组织建立的非致瘤性WI38细胞的生长，在相同的培养条件下未受到抑制；重组鼠γ干扰素（rMuIFN-γ）和HLT对鼠黑色素瘤B16具有相同的体外作用。因此，rMuIFN-γ与HLT联合使用可协同抑制B16细胞的生长，尽管单独使用任何一种淋巴因子孵育均无明显的抗增殖作用。对异步细胞的细胞周期进行流式细胞术分析表明，当B16黑色素瘤细胞用rMuIFN-γ和HLT联合处理时，与单独用rMuIFN-γ处理相比，更多细胞积聚在G0/G1期。似乎通常在细胞周期的S+G2+M期占很大比例的快速增殖肿瘤细胞群体，通过γ干扰素和HLT的联合作用而停滞在静止的G0/G1期，从而导致细胞生长受到抑制。
