Nagao S, Sato K, Ogawa H, Osada Y
Jpn J Cancer Res. 1986 Feb;77(2):212-8.
Stimulation by recombinant murine interferon-gamma (rMuIFN-gamma) of host defenses against the growth of Lewis lung adenocarcinoma, cell line 3LL, in the lung was examined. 3LL cells were resistant to in vitro antiproliferative activities of rMuIFN-gamma. On the other hand, rMuIFN-gamma augmented the killer activities of the non-adherent population of spleen cells and peripheral blood mononuclear cells (PBMC) in vivo, and the IFN also stimulated the cytostatic activities of peritoneal and splenic macrophages, but not alveolar macrophages. The combination of rMuIFN-gamma with LPS synergistically activated macrophages from the lung, as well as the peritoneal cavity and the spleen, to become both cytostatic and cytolytic against 3LL cells. The macrophages activated in vitro by simultaneous incubation with these agents markedly suppressed the growth of 3LL cells in vivo. The growth in the lung of 3LL cells implanted iv was significantly (P less than 0.05) suppressed by combination therapy with rMuIFN-gamma and LPS, but not by either agent alone. These results indicate that the potency of rMuIFN-gamma action against 3LL cells can be augmented by combination with LPS, mainly through synergistic macrophage activation.
研究了重组鼠γ干扰素（rMuIFN-γ）对宿主抵抗Lewis肺癌3LL细胞系在肺部生长的防御机制的刺激作用。3LL细胞对rMuIFN-γ的体外抗增殖活性具有抗性。另一方面，rMuIFN-γ在体内增强了脾细胞和外周血单核细胞（PBMC）非贴壁群体的杀伤活性，并且该干扰素还刺激了腹腔和脾巨噬细胞的细胞抑制活性，但未刺激肺泡巨噬细胞。rMuIFN-γ与LPS联合使用可协同激活来自肺以及腹腔和脾脏的巨噬细胞，使其对3LL细胞具有细胞抑制和细胞溶解作用。通过与这些试剂同时孵育在体外激活的巨噬细胞显著抑制了3LL细胞在体内的生长。静脉注射植入的3LL细胞在肺部的生长通过rMuIFN-γ和LPS联合治疗得到显著（P<0.05）抑制，但单独使用任何一种试剂均无此效果。这些结果表明，rMuIFN-γ与LPS联合使用可增强其对3LL细胞的作用效力，主要是通过协同激活巨噬细胞实现的。
