Sinha B K
Biochem Pharmacol. 1984 Sep 1;33(17):2777-81. doi: 10.1016/0006-2952(84)90695-6.
The metabolism of procarbazine was studied using spin-trapping techniques. The oxidation of procarbazine, catalyzed by horseradish peroxidase, prostaglandin synthetase [ram seminal vesicle (RSV) microsomes] or rat hepatic microsomal cytochrome P-450, produced carbon-centered free radicals. Cytochrome P-450 also catalyzed this oxidation in the presence of hydrogen peroxide. Horseradish peroxidase activation of procarbazine formed both the methyl radical and the N-isopropylbenzylamide radical [(CH3)2CHNHCO(C6H4)CH2.]. In the presence of RSV or rat hepatic microsomes, mostly the benzyl-type radical was trapped, presumably due to the reactivity of the methyl radical.
使用自旋捕捉技术研究了丙卡巴肼的代谢。辣根过氧化物酶、前列腺素合成酶[大鼠精囊（RSV）微粒体]或大鼠肝微粒体细胞色素P-450催化的丙卡巴肼氧化产生了以碳为中心的自由基。在过氧化氢存在下，细胞色素P-450也催化这种氧化反应。辣根过氧化物酶激活丙卡巴肼形成了甲基自由基和N-异丙基苄酰胺自由基[（CH3）2CHNHCO（C6H4）CH2·]。在RSV或大鼠肝微粒体存在的情况下，主要捕获的是苄基型自由基，这可能是由于甲基自由基的反应性所致。
