Butler R C
Br J Exp Pathol. 1984 Aug;65(4):509-19.
The role played by humoral and cellular immune response to liver antigens in the pathogenesis of experimental chronic active hepatitis (CAH) was studied in rabbits which had been immunized with the two human liver-specific proteins LSP and LP2, with LSP alone, or with an extract of human skeletal muscle. Rabbits immunized with LSP, alone or with LP2, developed skin test reactivity and circulating antibody to homologous LSP; liver biopsy revealed immunoglobulin bound to the hepatocyte cell surface. It has been suggested that cellular immunity to homologous LSP or alternatively, antibody to an antigenic determinant shared by human and rabbit LSP, may play a role in the pathogenesis of experimental CAH, but both abnormalities were present in two rabbits which did not develop CAH despite observation for at least 18 months. Five normal rabbits given an intravenous injection of serum pooled from rabbits with CAH did not develop significant hepatic lesions. Immunity to homologous LSP or other hepatocyte cell surface antigens could not be detected in any rabbit which had been immunized with skeletal muscle, and hepatocytes from these rabbits did not have immunoglobulin on their cell surface. The pathogenesis of CAH in these animals is obscure. These findings suggest that mechanisms other than an auto-immune response to LSP play an important role in the pathogenesis of experimental chronic active hepatitis.
在实验性慢性活动性肝炎（CAH）发病机制中，体液免疫和细胞免疫对肝脏抗原的反应所起的作用，在已用两种人肝脏特异性蛋白LSP和LP2、单独用LSP或用人骨骼肌提取物免疫的兔子中进行了研究。单独用LSP或与LP2一起免疫的兔子，出现了对同源LSP的皮肤试验反应性和循环抗体；肝活检显示免疫球蛋白结合在肝细胞表面。有人提出，对同源LSP的细胞免疫，或者对人和兔LSP共有的抗原决定簇的抗体，可能在实验性CAH的发病机制中起作用，但在两只尽管观察至少18个月仍未发生CAH的兔子中，这两种异常情况都存在。五只静脉注射来自患CAH兔子的混合血清的正常兔子，未出现明显的肝脏病变。在用骨骼肌免疫的任何兔子中，均未检测到对同源LSP或其他肝细胞表面抗原的免疫反应，这些兔子的肝细胞表面也没有免疫球蛋白。这些动物中CAH的发病机制尚不清楚。这些发现表明，除了对LSP的自身免疫反应外，其他机制在实验性慢性活动性肝炎的发病机制中起重要作用。
