Girard N, Maunoury R, Courel M N, Delpech B
C R Acad Sci III. 1984;298(18):517-22.
Splenic Mice cells immunized with glial fibrillary acidic protein (GFAP) were fused with SP 2/0 myeloma cells. After screening and cloning we obtained two types of hybridomas. Some of them secrete IgG class antibodies, the others IgM class antibodies. The specificity of these antibodies has been tested by three immunoenzymatic methods. The results are that IgG monoclonal antibodies identify an astrocyte-GFAP specific epitope and IgM monoclonal antibodies cross-react with a common epitope to GFAP and vimentin.
用胶质纤维酸性蛋白（GFAP）免疫的脾小鼠细胞与SP 2/0骨髓瘤细胞融合。经过筛选和克隆，我们获得了两种类型的杂交瘤。其中一些分泌IgG类抗体，另一些分泌IgM类抗体。这些抗体的特异性已通过三种免疫酶法进行了检测。结果是，IgG单克隆抗体识别星形胶质细胞-GFAP特异性表位，而IgM单克隆抗体与GFAP和波形蛋白的共同表位发生交叉反应。
