Linke R P, Hol P R, Gruys E, Geisel O, Nathrath W B, Trautwein G
J Comp Pathol. 1984 Jul;94(3):339-56. doi: 10.1016/0021-9975(84)90022-7.
Antisera were prepared in rabbits, sheep or chicken against purified amyloid fibril protein AA from man, mouse, stone marten, dog, cow and hamster. These antisera were tested by immunodiffusion against all purified antigens and applied to tissue sections containing amyloid from man, mouse, hamster, guinea pig, rabbit, cat, dog, mink, stone marten, pine marten, cow and horse. The binding of the antibodies to amyloid in tissue sections was assessed by the indirect immunoperoxidase method. The strongest reactions in the immunodiffusion and immunohistochemical methods were found between amyloid deposits of members of a given species and an antibody raised against protein AA from the same species. In contrast to the lack of cross-reactivity in immunodiffusion (except in the mouse-man relationship), extensive cross-reactions were observed immunohistochemically in phylogenetically related species, e.g. between stone marten, pine marten and mink, or between hamster and mouse. However, cross-reactions were also observed in combinations such as man-mouse, man-dog, man-cat, mouse-horse, and dog-cow. In addition, individual antisera showed variations in immunohistochemical reactivity with amyloid deposits of different members of one given species. Moreover, antisera prepared in rabbits reacted more restrictedly than those prepared in sheep, while rabbit antisera against any AA-protein did not react with rabbit amyloid. Finally, the widest degree of cross-reactivity including almost all mammalian species investigated was observed with a chicken antiserum to human amyloid AA protein.
用来自人、小鼠、石貂、狗、牛和仓鼠的纯化淀粉样原纤维蛋白AA免疫兔、绵羊或鸡来制备抗血清。通过免疫扩散针对所有纯化抗原对这些抗血清进行检测，并将其应用于含有人、小鼠、仓鼠、豚鼠、兔、猫、狗、水貂、石貂、松貂、牛和马的淀粉样物质的组织切片。采用间接免疫过氧化物酶法评估抗体与组织切片中淀粉样物质的结合情况。在免疫扩散和免疫组织化学方法中，在给定物种成员的淀粉样沉积物与针对同一物种的蛋白AA产生的抗体之间发现了最强反应。与免疫扩散中缺乏交叉反应（小鼠与人的关系除外）相反，在系统发育相关的物种中，例如石貂、松貂和水貂之间，或仓鼠和小鼠之间，在免疫组织化学中观察到广泛的交叉反应。然而，在人 - 小鼠、人 - 狗、人 - 猫、小鼠 - 马和狗 - 牛等组合中也观察到了交叉反应。此外，个别抗血清在与一个给定物种的不同成员的淀粉样沉积物的免疫组织化学反应性上表现出差异。而且，兔制备的抗血清比绵羊制备的抗血清反应更具局限性，而针对任何AA蛋白的兔抗血清都不与兔淀粉样物质反应。最后，用针对人淀粉样AA蛋白的鸡抗血清观察到了最广泛程度的交叉反应，几乎涵盖了所有研究的哺乳动物物种。
