Yamamoto S, Yoshimoto T, Furukawa M, Horie T, Watanabe-Kohno S
J Allergy Clin Immunol. 1984 Sep;74(3 Pt 2):349-52. doi: 10.1016/0091-6749(84)90128-3.
The 5-lipoxygenases of guinea pig peritoneal polymorphonuclear leukocytes and of rat basophilic leukemia cells have been solubilized, purified partially by affinity chromatography, and shown to convert arachidonic acid principally to 5-hydroperoxy-6,8,11,14- eicosatetraenoic acid. The activity of both 5-lipoxygenases is calcium dependent and enhanced by adenosine triphosphate and other nucleotides in the presence of calcium ion. Both 5-lipoxygenase activity and leukotriene generation by sensitized guinea pig lung tissue challenged with antigen were suppressed substantially by specific benzoquinone and flavonoid inhibitors. The in vivo significance of the findings will be explored with potent and selective lipoxygenase inhibitors, which are delineated in the 5-lipoxygenase model systems.
豚鼠腹膜多形核白细胞和大鼠嗜碱性白血病细胞的5-脂氧合酶已被溶解，并通过亲和层析进行了部分纯化，结果表明它们可将花生四烯酸主要转化为5-氢过氧-6,8,11,14-二十碳四烯酸。两种5-脂氧合酶的活性均依赖于钙，并且在钙离子存在的情况下，三磷酸腺苷和其他核苷酸可增强其活性。用抗原攻击致敏的豚鼠肺组织时，特异性苯醌和类黄酮抑制剂可显著抑制5-脂氧合酶活性和白三烯的生成。将使用在5-脂氧合酶模型系统中描述的强效和选择性脂氧合酶抑制剂来探索这些发现的体内意义。
