Muir M E, van Heeswyck R S, Wallace B J
J Gen Microbiol. 1984 Aug;130(8):2015-22. doi: 10.1099/00221287-130-8-2015.
The rate of accumulation of streptomycin by streptomycin-sensitive strains of Escherichia coli and Bacillus megaterium, grown in chemostats, was related to the growth rate prior to addition of the antibiotic. For E. coli the length of the lag period that preceded accumulation was also growth rate-dependent. Thus faster growing cultures accumulated streptomycin more rapidly and with a shorter lag than slower growing cultures. The rate of efflux of streptomycin from bacteria that had accumulated streptomycin was not greatly influenced by growth rates of the cultures. At a particular growth rate, accumulation of streptomycin was found to be faster at higher concentrations of the antibiotic. Rapid accumulation of streptomycin was not observed with continuous cultures of a streptomycin-resistant strain of E. coli. Accumulation of streptomycin was abolished when growth was inhibited by either terminating the flow of fresh medium to a chemostat or by adding inhibitors that block protein synthesis. These results suggest that the rate of accumulation of streptomycin is related to the concentration of streptomycin-sensitive ribosomes that are actively engaged in protein synthesis within the bacterial cells.
在恒化器中培养的对链霉素敏感的大肠杆菌和巨大芽孢杆菌菌株积累链霉素的速率，与添加抗生素之前的生长速率相关。对于大肠杆菌，积累之前的延迟期长度也取决于生长速率。因此，生长较快的培养物比生长较慢的培养物积累链霉素的速度更快，延迟期更短。已积累链霉素的细菌中链霉素的流出速率受培养物生长速率的影响不大。在特定生长速率下，发现抗生素浓度较高时链霉素的积累更快。对链霉素耐药的大肠杆菌连续培养物未观察到链霉素的快速积累。当通过终止向恒化器中流入新鲜培养基或添加阻断蛋白质合成的抑制剂来抑制生长时，链霉素的积累就会停止。这些结果表明，链霉素的积累速率与细菌细胞内积极参与蛋白质合成的链霉素敏感核糖体的浓度有关。
