Schuster G S, Singh B B, Erbland J F, Ringler M B
J Oral Pathol. 1984 Aug;13(4):382-9. doi: 10.1111/j.1600-0714.1984.tb01437.x.
TPA, a tumor promotor whose initial site of action is the cell membrane, was examined for its actions on hamster oral mucosa using light and scanning electron-microscopic procedures. Hamster cheek-pouch explants were cultured in vitro, then treated for 72 h with 1.6 X 10(-8) M TPA. Treated cultures showed a higher mitotic index and more extensive growth than control cultures. Epithelial cells in the control cultures appeared polygonal, with thin, small to medium microvilli. The cells in the treated cultures showed variable shape and surface morphology. Some were interconnected by broad cytoplasmic extensions while others demonstrated long, thin processes that traversed great distances. The unusual surface morphology may be a manifestation of altered phospholipid metabolism that produces a more "fluid" cytoplasmic membrane.
佛波酯（TPA）是一种肿瘤促进剂，其初始作用位点是细胞膜，我们使用光学显微镜和扫描电子显微镜技术研究了它对仓鼠口腔黏膜的作用。将仓鼠颊囊外植体进行体外培养，然后用1.6×10⁻⁸ M的佛波酯处理72小时。与对照培养物相比，经处理的培养物显示出更高的有丝分裂指数和更广泛的生长。对照培养物中的上皮细胞呈多边形，具有细小至中等的微绒毛。经处理的培养物中的细胞呈现出可变的形状和表面形态。一些细胞通过宽阔的细胞质延伸相互连接，而另一些则表现出长而细的突起，这些突起延伸很长距离。这种异常的表面形态可能是磷脂代谢改变的表现，这种改变产生了一种更“流动”的细胞质膜。
