Umezawa K, Fujie S
Gan. 1980 Dec;71(6):871-4.
The growth on hamster embryonic fibroblasts was highly dependent on the serum concentration in the medium. A tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), enhanced the growth of hamster embryonic fibroblasts in the medium containing low concentrations of serum. Among phorbol derivatives, phorbol-12,13-didecanoate and 4-O-methyl-12-O-tetradecanoyl-phorbol-13-acetate also showed growth stimulation, but 4 alpha-phorbol-12,13-didecanoate, phorbol-12,13-diacetate and phorbol did not. TPA increased the accumulation of 2-deoxyglucose into the cells at low serum concentrations. Thus, hamster embryonic fibroblasts were sensitive to phorbol esters as growth stimulators, and TPA reduced the serum requirement of the cells.
仓鼠胚胎成纤维细胞的生长高度依赖于培养基中的血清浓度。一种肿瘤促进剂，12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA），在含有低浓度血清的培养基中增强了仓鼠胚胎成纤维细胞的生长。在佛波醇衍生物中，佛波醇 - 12,13 - 二癸酸酯和4 - O - 甲基 - 12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯也显示出促生长作用，但4α - 佛波醇 - 12,13 - 二癸酸酯、佛波醇 - 12,13 - 二乙酸酯和佛波醇则没有。在低血清浓度下，TPA增加了2 - 脱氧葡萄糖在细胞内的积累。因此，仓鼠胚胎成纤维细胞对佛波醇酯作为生长刺激剂敏感，并且TPA降低了细胞对血清的需求。
