儿童小肠活检组织中的二胺氧化酶和双糖酶活性

Diamine oxidase and disaccharidase activities in small intestinal biopsies of children.

作者信息

Forget P, Grandfils C, van Cutsem J L, Dandrifosse G

出版信息

Pediatr Res. 1984 Jul;18(7):647-9. doi: 10.1203/00006450-198407000-00016.

DOI:10.1203/00006450-198407000-00016

Abstract

A relationship between disaccharidase and diamine oxidase (DAO) activities was looked for by measuring these enzyme activities in histologically normal small intestinal biopsies of 18 children. The range for disaccharidase activities expressed in U g-1 wet weight was 0.1-5.7, 7.1-36.7, and 2.3-8.5 for lactase, maltase, and sucrase, respectively. The range for DAO activities expressed in nmol h-1 g-1 wet weight was 202-974. Significant correlations were found between disaccharidase and DAO activities (lactase versus DAO: n = 17, r = 0.80, P less than 0.001; maltase versus DAO: n = 18, r = 0.70, P less than 0.001; sucrase versus DAO: n = 18, r = 0.55, P less than 0.05). Our results further support the hypothesis that DAO is a marker of small intestinal functional integrity in children.

摘要

通过检测18名儿童组织学正常的小肠活检标本中的这些酶活性，探寻二糖酶与二胺氧化酶（DAO）活性之间的关系。以每克湿重的酶活性单位（U）表示，乳糖酶、麦芽糖酶和蔗糖酶的二糖酶活性范围分别为0.1 - 5.7、7.1 - 36.7和2.3 - 8.5。以每小时每克湿重的纳摩尔数（nmol h-1 g-1）表示，DAO活性范围为202 - 974。发现二糖酶与DAO活性之间存在显著相关性（乳糖酶与DAO：n = 17，r = 0.80，P < 0.001；麦芽糖酶与DAO：n = 18，r = 0.70，P < 0.001；蔗糖酶与DAO：n = 18，r = 0.55，P < 0.05）。我们的结果进一步支持了DAO是儿童小肠功能完整性标志物的假说。