Kolho K L, Savilahti E
Hospital for Children and Adolescents, Helsinki University Central Hospital, Finland.
J Pediatr Gastroenterol Nutr. 2000 Mar;30(3):283-7. doi: 10.1097/00005176-200003000-00013.
Intestinal disaccharidase activities tend to be low in villous atrophy, but there are only a few reports of enzyme activities in children with normal villous architecture.
In the current study the data were reviewed on disaccharidase activities in duodenal biopsy specimens of normal villous structure in 223 children undergoing upper gastrointestinal endoscopy in 1997 and 1998. The ancestry was Finnish in 188 children (median age 8.0 years; range, 0.2-18 years), African in 27 children (median age 5.0 years; range, 1-13 years), and other in eight children.
The mean activities of lactase, sucrase, and maltase were significantly higher in Finnish children than in children of African origin (P < 0.0001, P < 0.002, and P < 0.02, respectively). Lactase activity decreased with increasing age (P < 0.001), but age had no significant effect on maltase and sucrase activities. Among Finnish children, 31% (59/188) had lactase activity below the established reference range of 20 units (units are micromoles of substrate hydrolyzed per minute at 37 degrees C per gram of protein) and one child had a probable sucrase-isomaltase deficiency. When these 60 children with low enzyme activities were excluded, the geometric means were lactase, 35.7 units (95% confidence interval [CI], 32.8-38.6 units); maltase, 241 units (95% CI, 225-258 units); and sucrase, 57.5 units (95% CI, 53.5-61.6 units). Among the children of African origin, lactase activity was decreased in 67% (18/27). All three enzyme activities were decreased in parallel more often among the African children (8/27) than among the Finnish children (9/188; P < 0.002).
Ethnicity has a strong effect on disaccharidase values in children with normal villous structure. African children have lower activities of lactase, sucrase, and maltase in duodenal specimens than do children of Finnish origin.
绒毛萎缩时肠双糖酶活性往往较低，但关于绒毛结构正常的儿童酶活性的报道较少。
在本研究中，回顾了1997年和1998年接受上消化道内镜检查的223名儿童十二指肠活检标本中双糖酶活性的数据。其中188名儿童为芬兰裔（中位年龄8.0岁；范围0.2 - 18岁），27名儿童为非洲裔（中位年龄5.0岁；范围1 - 13岁），8名儿童为其他族裔。
芬兰儿童的乳糖酶、蔗糖酶和麦芽糖酶平均活性显著高于非洲裔儿童（分别为P < 0.0001、P < 0.002和P < 0.02）。乳糖酶活性随年龄增长而降低（P < 0.001），但年龄对麦芽糖酶和蔗糖酶活性无显著影响。在芬兰儿童中，31%（59/188）的乳糖酶活性低于既定参考范围20单位（单位为每克蛋白质在37℃每分钟水解底物的微摩尔数），1名儿童可能存在蔗糖酶 - 异麦芽糖酶缺乏。排除这60名酶活性低的儿童后，几何平均数为：乳糖酶35.7单位（95%置信区间[CI]，32.8 - 38.6单位）；麦芽糖酶241单位（95% CI，225 - 258单位）；蔗糖酶57.5单位（95% CI，53.5 - 61.6单位）。在非洲裔儿童中，67%（18/27）的乳糖酶活性降低。与芬兰儿童（9/188）相比，非洲儿童中三种酶活性同时降低的情况更常见（8/27；P < 0.002）。
种族对绒毛结构正常的儿童双糖酶值有很大影响。非洲儿童十二指肠标本中乳糖酶、蔗糖酶和麦芽糖酶的活性低于芬兰裔儿童。
