二价阳离子通过C3受体对玫瑰花结形成的影响。

The effect of divalent cations on the rosette formation via C3 receptors.

作者信息

Okuda T, Sato S, Tachibana T

出版信息

Tohoku J Exp Med. 1984 Jun;143(2):221-9. doi: 10.1620/tjem.143.221.

DOI:10.1620/tjem.143.221

Abstract

The indicator cells with the specificity for the various C3 receptors were prepared using highly purified complement components. The effect of EDTA and divalent cations on the rosette formation of these indicators with human blood cells and lymphoblastoid cells were investigated. B lymphocytes form rosettes with EACl-3b, EACl-3bH, EACl-3bi and EACl-3d irrespective of the presence of cations. PMN and monocytes form rosettes with EACl-3b and EACl-3bH independent of cations, but their formation with EAC1-3bi needs the presence of magnesium. In this case calcium shows a cooperative effect with magnesium. PMN and monocytes do not react with EACl-3d even in the presence of magnesium. Although Raji cells do not react with EACl-3b, they react with EACl-3bH independent of divalent cations. EACm has a similar reactivity to EACl-3bi, and by the treatment with trypsin it becomes similar to EACl-3d.

摘要

使用高度纯化的补体成分制备了对各种C3受体具有特异性的指示细胞。研究了EDTA和二价阳离子对这些指示细胞与人类血细胞和淋巴母细胞形成花环的影响。B淋巴细胞与EAC1-3b、EAC1-3bH、EAC1-3bi和EAC1-3d形成花环，与阳离子的存在无关。PMN和单核细胞与EAC1-3b和EAC1-3bH形成花环，与阳离子无关，但它们与EAC1-3bi的形成需要镁的存在。在这种情况下，钙与镁表现出协同作用。即使在镁存在的情况下，PMN和单核细胞也不与EAC1-3d反应。虽然Raji细胞不与EAC1-3b反应，但它们与EAC1-3bH反应，与二价阳离子无关。EACm与EAC1-3bi具有相似的反应性，通过胰蛋白酶处理后变得与EAC1-3d相似。