Newman S L, Devery-Pocius J E, Ross G D, Henson P M
Complement. 1984;1(4):213-27.
Human monocytes and monocyte-derived macrophages were examined for their ability to bind and ingest C3-coated sheep erythrocytes (E). Greater than 90% of monocytes and macrophages formed rosettes with EC3b and EC3bi prepared with 15,000-20,000 molecules of C3 per E. Binding of EC3b to the monocyte or macrophage surface was inhibited by rabbit anti-C3b receptor (CR1), but was not inhibited by two different monoclonal anti-C3bi receptor (CR3) antibodies. EC3bi rosette formation was inhibited by monoclonal anti-CR3, but not by anti-CR1. Monocytes and macrophages did not form rosettes with similarly prepared EC3d,g or EC3d. Macrophages cultured for 7 days, but not freshly isolated monocytes, phagocytosed both EC3b and EC3bi. This ability was a consequence of macrophage maturation, as no external stimuli were present during in vitro culture. Experiments directed to determine if EC3b was converted to EC3bi before ingestion suggested that macrophage CR1 and CR3 mediated phagocytosis independently. No evidence was obtained that during the phagocytosis assay, macrophage factor I converted EC3b to EC3bi. The number of E bound or ingested by monocytes and macrophages was dependent on the number of molecules of C3b or iC3b bound per E. Monocytes and macrophages did not require the presence of either Ca++ or Mg++ for rosette formation with EC3b, whereas both divalent cations were required for optimum rosette formation with EC3bi. The presence of divalent cations was required for macrophage phagocytosis of EC3b and EC3bi. For ingestion of EC3b, Mg++ alone was sufficient, and the addition of Ca++ did not increase the number of EC3b ingested. For ingestion of EC3bi, both Ca++ and Mg++ were required for optimal phagocytosis, and their effect was concentration dependent and additive.
对人单核细胞和单核细胞衍生的巨噬细胞结合和摄取C3包被的绵羊红细胞（E）的能力进行了检测。超过90%的单核细胞和巨噬细胞与每E结合15,000 - 20,000个C3分子制备的EC3b和EC3bi形成花环。EC3b与单核细胞或巨噬细胞表面的结合被兔抗C3b受体（CR1）抑制，但不被两种不同的抗C3bi受体（CR3）单克隆抗体抑制。EC3bi花环形成被抗CR3单克隆抗体抑制，但不被抗CR1抑制。单核细胞和巨噬细胞不与同样制备的EC3d,g或EC3d形成花环。培养7天的巨噬细胞，而非新鲜分离的单核细胞，吞噬EC3b和EC3bi。这种能力是巨噬细胞成熟的结果，因为体外培养期间不存在外部刺激。旨在确定EC3b在摄取前是否转化为EC3bi的实验表明，巨噬细胞CR1和CR3独立介导吞噬作用。未获得证据表明在吞噬试验期间，巨噬细胞I因子将EC3b转化为EC3bi。单核细胞和巨噬细胞结合或摄取的E的数量取决于每E结合的C3b或iC3b分子数量。单核细胞和巨噬细胞与EC3b形成花环不需要Ca++或Mg++的存在，而与EC3bi形成最佳花环需要两种二价阳离子。二价阳离子的存在是巨噬细胞吞噬EC3b和EC3bi所必需的。对于EC3b的摄取，单独的Mg++就足够了，添加Ca++不会增加摄取的EC3b数量。对于EC3bi的摄取，Ca++和Mg++都是最佳吞噬作用所必需的，它们的作用是浓度依赖性的且具有加和性。
