用L-抗坏血酸进行电子显微镜检查的脱钙处理。
Decalcification for electron microscopy with L-ascorbic acid.
作者信息
Wakita M, Kobayashi M, Shioi T
出版信息
Stain Technol. 1983 Nov;58(6):337-41. doi: 10.3109/10520298309066809.
PMID:6433515
Abstract
L-Ascorbic acid decalcification was used for electron microscopy of mammalian tooth germs and bone after fixation in a glutaraldehyde-paraformaldehyde mixture. The recommended decalcifying solution is 2% with respect to L-ascorbic acid and 0.9% with respect to sodium chloride. The method has the advantage that decalcification is complete within a quarter of the time required with EDTA. The fine structure of ameloblasts and hard tissue is preserved as well as with EDTA.
摘要
在戊二醛 - 多聚甲醛混合液中固定后，采用L - 抗坏血酸脱钙法对哺乳动物牙胚和骨骼进行电子显微镜观察。推荐的脱钙溶液中L - 抗坏血酸含量为2%，氯化钠含量为0.9%。该方法的优点是脱钙时间仅为乙二胺四乙酸（EDTA）所需时间的四分之一，且能像使用EDTA一样很好地保存成釉细胞和硬组织的精细结构。
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