一种用于棘皮动物组织超微结构的脱钙方法。
A decalification method for ultrastructure of echinoderm tissues.
作者信息
Dietrich H F, Fontaine A R
出版信息
Stain Technol. 1975 Sep;50(5):351-4. doi: 10.3109/10520297509117086.
PMID:813335
Abstract
Decalcification of echinoderm tissues for electron microscopy can be achieved after glutaraldehyde-osmium fixation by treatment with a 1:1 mixture of 2% ascorbic acid and 0.3 M NaCl for 12-24 hours. Electron photomicrographs of material decalcified by this procedure are superior to those from EDTA-treated tissue and show few of the deleteriour effects produced by EDTA.
摘要
通过戊二醛-锇固定后，用2%抗坏血酸和0.3M氯化钠的1:1混合物处理12 - 24小时，可实现用于电子显微镜观察的棘皮动物组织脱钙。用此方法脱钙的材料的电子显微照片优于经乙二胺四乙酸（EDTA）处理的组织的照片，并且显示出由EDTA产生的很少有害影响。
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