Kreslavskaia E E, Chibisov I V, Antipova R I, Narycheva I A
Vopr Med Khim. 1984 May-Jun;30(3):128-31.
Homogeneous preparation of acid alpha-glucosidase, obtained from human placenta, was used for immunization of rabbits and production of specific antiserum. Immunological procedure was developed for estimation of acid alpha-glucosidase activity from human muscles in presence of the enzyme neutral form. Activity of acid alpha-glucosidase was measured in 20 patients with various types of nervous system impairments. Total activity of alpha-glucosidase in muscles of the patients was 11.4 +/- 0.7 nM/min/g of the tissue and activity of the acid form constituted about 84.8 +/- 1.7% of the total activity.
从人胎盘中获得的酸性α-葡萄糖苷酶的均一制剂用于免疫兔子并制备特异性抗血清。开发了免疫程序以在酶的中性形式存在下估计人肌肉中酸性α-葡萄糖苷酶的活性。在20名患有各种类型神经系统损伤的患者中测量了酸性α-葡萄糖苷酶的活性。患者肌肉中α-葡萄糖苷酶的总活性为11.4±0.7 nM/分钟/克组织，酸性形式的活性约占总活性的84.8±1.7%。
