寡糖修剪酶葡萄糖苷酶II的免疫定位
Immunolocalization of the oligosaccharide trimming enzyme glucosidase II.
作者信息
Lucocq J M, Brada D, Roth J
出版信息
J Cell Biol. 1986 Jun;102(6):2137-46. doi: 10.1083/jcb.102.6.2137.
Abstract
We used immunoelectron microscopy to localize glucosidase II in pig hepatocytes. The enzyme trims the two inner alpha 1,3-linked glucoses from N-linked oligosaccharide precursor chains of glycoproteins. Immunoreactive enzyme was concentrated in rough (RER) and smooth (SER) endoplasmic reticulum but not detectable in Golgi apparatus cisternae. Transitional elements of RER and smooth membraned structures close to Golgi apparatus cisternae contained labeling for glucosidase II. Specific labeling was also found in autophagosomes. These results indicate strongly that glucosidase II acts on glycoproteins before their transport to, and processing in Golgi apparatus cisternae, and suggest that an important transitional region for glucosidase II exists between RER and Golgi apparatus cisternae. Degradation in autophagolysosomes could form a normal catabolic pathway for glucosidase II.
摘要
我们利用免疫电子显微镜对猪肝细胞中的葡萄糖苷酶II进行定位。该酶从糖蛋白的N - 连接寡糖前体链上切除两个内部的α1,3 - 连接葡萄糖。免疫反应性酶集中在粗面内质网（RER）和滑面内质网（SER）中，但在高尔基器潴泡中未检测到。靠近高尔基器潴泡的粗面内质网过渡元件和滑面膜结构含有葡萄糖苷酶II的标记。在自噬体中也发现了特异性标记。这些结果有力地表明，葡萄糖苷酶II在糖蛋白转运至高尔基器潴泡并在其中加工之前就作用于糖蛋白，并提示在粗面内质网和高尔基器潴泡之间存在一个葡萄糖苷酶II的重要过渡区域。自噬溶酶体中的降解可能构成葡萄糖苷酶II的正常分解代谢途径。
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