Chandler D K, Barile M F
Yale J Biol Med. 1983 Sep-Dec;56(5-6):679-83.
Attachment of radiolabeled M. pneumoniae to human WiDr cell culture monolayers was dependent on the WiDr cell density and the concentration of M. pneumoniae. Saturation of confluent monolayers grown on 5 mm coverslips was attained with only 40 micrograms of M. pneumoniae protein. Preincubating the WiDr monolayers with unlabeled M. pneumoniae or with a protein-rich extract prepared from M. pneumoniae inhibited subsequent attachment of radiolabeled organisms. Attachment inhibition by the M. pneumoniae extract provided a quantitative assay for mycoplasmal binding components. Treatment of radiolabeled M. pneumoniae with orosomucoid, ceruloplasmin, and gangliosides inhibited attachment to WiDr cells. These sialoglycoconjugates may be structural analogues of the target cell receptor.
放射性标记的肺炎支原体附着于人类WiDr细胞培养单层，这取决于WiDr细胞密度和肺炎支原体的浓度。在5毫米盖玻片上生长的汇合单层细胞，仅用40微克肺炎支原体蛋白即可达到饱和。用未标记的肺炎支原体或从肺炎支原体制备的富含蛋白质的提取物对WiDr单层细胞进行预孵育，可抑制随后放射性标记生物体的附着。肺炎支原体提取物的附着抑制作用为支原体结合成分提供了一种定量测定方法。用类粘蛋白、铜蓝蛋白和神经节苷脂处理放射性标记的肺炎支原体可抑制其对WiDr细胞的附着。这些唾液酸糖缀合物可能是靶细胞受体的结构类似物。
