Chandler D K, Razin S, Stephens E B, Harasawa R, Barile M F
Infect Immun. 1982 Nov;38(2):604-9. doi: 10.1128/iai.38.2.604-609.1982.
Mycoplasma pneumoniae strains PI-1428, M129, B176, FH, and MAC were analyzed for homology by DNA-DNA hybridization, thermal elution midpoints of the DNA-DNA heteroduplexes, DNA cleavage patterns with restriction endonucleases, and protein gel electrophoretic profiles. These properties were compared with biological assays, such as antigenic reactivity with reference antisera in the growth inhibition and metabolic inhibition tests, as well as the ability to attach to human WiDr cell cultures. The avirulent, nonhemadsorbing strain B176 could be differentiated from the others by diminished attachment capacity to WiDr cells and by slightly reduced DNA homology. The other strains appeared to be identical when examined by these procedures. No significant differences were detected among any of the strains by the metabolic inhibition or growth inhibition tests. The DNA cleavage patterns of the five strains were also similar, although strains FH and MAC differed from the other three strains in a few bands. These results demonstrate that these five strains are similar and that the species M. pneumoniae is remarkably homogeneous.
通过DNA-DNA杂交、DNA-DNA异源双链体的热洗脱中点、限制性内切酶的DNA切割模式以及蛋白质凝胶电泳图谱，对肺炎支原体菌株PI-1428、M129、B176、FH和MAC进行了同源性分析。将这些特性与生物学测定进行了比较，如在生长抑制和代谢抑制试验中与参考抗血清的抗原反应性，以及附着于人类WiDr细胞培养物的能力。无毒、无血细胞吸附的菌株B176可通过对WiDr细胞的附着能力降低以及DNA同源性略有降低与其他菌株区分开来。通过这些程序检查时，其他菌株似乎是相同的。在代谢抑制或生长抑制试验中，未在任何菌株之间检测到显著差异。这五个菌株的DNA切割模式也相似，尽管FH和MAC菌株在一些条带上与其他三个菌株不同。这些结果表明，这五个菌株相似，并且肺炎支原体物种非常同质。
