Demoulin A, Lambotte R, Franchimont P
Acta Eur Fertil. 1984 Mar-Apr;15(2):99-109.
Increasing concentrations of estradiol, testosterone, progesterone (1.10(-10) to 1.10(-7] and Danazol (1.10(-9) to 1.10(-6) M) have been added to male rat pituitary cells maintained in monolayer cultures for a preincubation period of three days followed by a six hour incubation with or without GnRH (1.10(-8) M). Concentrations of LH and FSH have been assessed in the culture media and in the cells at the end of the experiments allowing an estimation of the influence of these steroids on gonadotropin release and synthesis. In these experimental conditions, estradiol does not modify basal and GnRH induced FSH release and synthesis but reduces the GnRH-induced response of LH. Testosterone and progesterone stimulate synthesis of FSH but inhibit synthesis and secretion of LH in the presence of GnRH. These results have been compared with those of literature. Danazol, in the same experimental conditions, stimulates synthesis of gonadotropins and simultaneously inhibits their release induced by the presence of GnRH. We conclude that Danazol is able to act at the pituitary level as testosterone which is in good agreement with its androgenic properties.
已将递增浓度的雌二醇、睾酮、孕酮（1.10(-10) 至 1.10(-7]）和达那唑（1.10(-9) 至 1.10(-6) M）添加到单层培养的雄性大鼠垂体细胞中，预孵育三天，然后在有或无促性腺激素释放激素（GnRH，1.10(-8) M）的情况下孵育六小时。在实验结束时评估了培养基和细胞中促黄体生成素（LH）和促卵泡生成素（FSH）的浓度，从而可以估计这些类固醇对促性腺激素释放和合成的影响。在这些实验条件下，雌二醇不会改变基础状态下以及GnRH诱导的FSH释放和合成，但会降低GnRH诱导的LH反应。睾酮和孕酮在存在GnRH的情况下刺激FSH的合成，但抑制LH的合成和分泌。已将这些结果与文献中的结果进行了比较。在相同实验条件下，达那唑刺激促性腺激素的合成，同时抑制由GnRH存在所诱导的促性腺激素释放。我们得出结论，达那唑在垂体水平上的作用与睾酮相似，这与其雄激素特性相符。
