Kugu K, Kohda K, Taketani Y, Mizuno M
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, Japan.
Arch Gynecol Obstet. 1989;244(3):157-62. doi: 10.1007/BF00931293.
The actions of danazol on the release of gonadotropins and prolactin (PRL) were investigated by using a culture of rat anterior pituitary gland cells. The addition of danazol in the range between 10(-9) M and 10(-5) M elevated the levels of follicle-stimulating hormone (FSH) in a dose-dependent manner. Danazol at 10(-5) M caused a 70% increase in FSH levels over the control. The intracellular contents of FSH was also increased by danazol, suggesting its stimulatory effect on both the synthesis and release of FSH. Danazol had no effect on the release of luteinizing hormone (LH). Danazol diminished the sensitivity of gonadotrophs to luteinizing hormone-releasing hormone (LHRH) in that the LHRH-induced release of both FSH and LH was suppressed. The amount of PRL released into medium was decreased by danazol in a dose-dependent way.
通过使用大鼠垂体前叶腺细胞培养物，研究了达那唑对促性腺激素和催乳素（PRL）释放的作用。在10^(-9)M至10^(-5)M范围内添加达那唑，以剂量依赖的方式提高了促卵泡激素（FSH）的水平。10^(-5)M的达那唑使FSH水平比对照增加了70%。达那唑还增加了FSH的细胞内含量，表明其对FSH的合成和释放均有刺激作用。达那唑对促黄体生成素（LH）的释放没有影响。达那唑降低了促性腺细胞对促黄体生成素释放激素（LHRH）的敏感性，因为LHRH诱导的FSH和LH释放均受到抑制。达那唑以剂量依赖的方式减少了释放到培养基中的PRL量。
