Lim Y H, Gould M K
Biochem Int. 1983 Feb;6(2):163-9.
Muscle ATP levels were lowered by incubating rat soleus muscles under anaerobic conditions, or in the presence of 2:4-dinitrophenol (0.5 mM), EDTA (5 mM) or mannitol (400 mM). 125I-insulin binding, measured under equilibrium conditions at 25 degrees C, was reduced by 49-71% in ATP-depleted muscles. Insulin binding was also determined using two other procedures which minimized internalization of 125I-insulin: these were (a) 5 min at 25 degrees C, and (b) 24 h at 3 degrees C. Under these conditions, 125I-insulin binding was reduced by 28-55% in ATP-depleted muscles. These results confirm that in soleus muscle the effect of ATP-depletion on 125I-insulin binding is actually concerned with the binding step itself and not merely a reflection of ATP-dependent internalization of the bound hormone.
通过在无氧条件下孵育大鼠比目鱼肌，或在存在2,4-二硝基苯酚（0.5 mM）、乙二胺四乙酸（5 mM）或甘露醇（400 mM）的情况下，肌肉中的三磷酸腺苷（ATP）水平会降低。在25摄氏度的平衡条件下测量的125I胰岛素结合，在ATP耗尽的肌肉中减少了49%-71%。还使用了另外两种将125I胰岛素内化降至最低的方法来测定胰岛素结合：这些方法是（a）在25摄氏度下5分钟，以及（b）在3摄氏度下24小时。在这些条件下，ATP耗尽的肌肉中125I胰岛素结合减少了28%-55%。这些结果证实，在比目鱼肌中，ATP耗尽对125I胰岛素结合的影响实际上与结合步骤本身有关，而不仅仅是结合激素的ATP依赖性内化的反映。
