Tanaka C, Taniyama K, Kusunoki M
FEBS Lett. 1984 Sep 17;175(1):165-9. doi: 10.1016/0014-5793(84)80591-8.
Electrical stimulation of guinea pig ileum preloaded with [3H]choline provokes the release of [3H]acetylcholine (ACh) in a Ca2+-dependent manner. This release was markedly increased by the tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA). The combination of the ionophore A23187 and TPA produced the release of [3H]ACh up to a level equal to or exceeding a maximal response induced by electrical stimulation. A23187 alone gave only a minor response and TPA alone had no apparent effect on the [3H]ACh release. Thus, protein kinase C probably plays a role in cell surface signal transduction related to the release of transmitters from nerve endings.
对预先加载了[3H]胆碱的豚鼠回肠进行电刺激，会以Ca2+依赖的方式引发[3H]乙酰胆碱（ACh）的释放。促肿瘤佛波酯12 - O - 十四烷酰佛波醇13 - 乙酸酯（TPA）可显著增加这种释放。离子载体A23187与TPA联合使用可使[3H]ACh的释放达到或超过电刺激诱导的最大反应水平。单独使用A23187仅有轻微反应，单独使用TPA对[3H]ACh释放无明显影响。因此，蛋白激酶C可能在与神经末梢递质释放相关的细胞表面信号转导中发挥作用。
