Hegre O D, Simeonovic C J, Lafferty K J
Diabetes. 1984 Oct;33(10):975-7. doi: 10.2337/diab.33.10.975.
Proislets, derived from fetal mouse pancreata by collagenase digestion and subsequent organ culture, can be frozen to -196 degrees C and stored in a viable condition before successful syngeneic transplantation. Cryopreserved proislets are relatively undifferentiated morphologically, but continue to differentiate into mature islets after transplantation.
通过胶原酶消化和随后的器官培养从胎鼠胰腺中获得的前胰岛,可以冷冻至-196℃并在成功的同基因移植前以存活状态储存。冷冻保存的前胰岛在形态上相对未分化,但在移植后会继续分化为成熟胰岛。