Herman R K
Genetics. 1984 Sep;108(1):165-80. doi: 10.1093/genetics/108.1.165.
A new method for producing genetic mosaics, which involves the spontaneous somatic loss of free chromosome fragments, is demonstrated. Four genes that affect the behavior of C. elegans were studied in mosaic animals. The analysis is known. Two of the mutant genes affect certain sensory responses and prevent uptake of fluorescein isothiocyanate (FITC) by certain sensory neurons. Mosaic analysis indicated that one of these mutant genes is cell autonomous with respect to its effect on FITC uptake and the other is cell nonautonomous. In the latter case, the genotype of a non-neuronal supporting cell that surrounds the processes of the neurons that normally take up FITC probably is critical. The other two mutant genes affect animal movement. Mosaic analysis indicated that the expression of one of these genes is specific to certain neurons (motor neurons of the ventral and dorsal nerve cords are prime candidates and the expression of the other gene is specific to muscle cells.
本文展示了一种产生基因嵌合体的新方法，该方法涉及游离染色体片段的自发体细胞丢失。在嵌合体动物中研究了四个影响秀丽隐杆线虫行为的基因。分析过程是已知的。其中两个突变基因影响特定的感觉反应，并阻止某些感觉神经元摄取异硫氰酸荧光素（FITC）。嵌合体分析表明，这些突变基因中的一个对FITC摄取的影响是细胞自主的，另一个则是非细胞自主的。在后一种情况下，围绕通常摄取FITC的神经元突起的非神经元支持细胞的基因型可能至关重要。另外两个突变基因影响动物运动。嵌合体分析表明，其中一个基因的表达特定于某些神经元（腹侧和背侧神经索的运动神经元是主要候选者），另一个基因的表达特定于肌肉细胞。
