观察和量化荧光报告基因。
Observing and Quantifying Fluorescent Reporters.
机构信息
Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, USA.
Department of Biology, McGill University, Montreal, QC, Canada.
出版信息
Methods Mol Biol. 2022;2468:73-87. doi: 10.1007/978-1-0716-2181-3_5.
Genetically encoded fluorescent reporters take advantage of C. elegans' transparency to allow non-invasive, in vivo observation, and recording of physiological processes in intact animals. Here, we discuss the basic microscope components required to observe, image, and measure fluorescent proteins in live animals for students and researchers who work with C. elegans but have limited experience with fluorescence imaging and analysis.
遗传编码荧光报告基因利用秀丽隐杆线虫的透明性，实现了对完整动物体内生理过程的非侵入式、活体观察和记录。在这里，我们为那些使用秀丽隐杆线虫但荧光成像和分析经验有限的学生和研究人员讨论了观察、成像和测量活体动物中荧光蛋白所需的基本显微镜组件。
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