Caraty A, Martin G B, Montgomery G
Reprod Nutr Dev (1980). 1984;24(4):439-48. doi: 10.1051/rnd:19840409.
In two experiments with ovariectomized ewes, the plasma levels of LH and FSH were measured following passive immunization against LH-RH. The pulsatile secretion of LH was inhibited and the levels of the hormone fell quickly and were undetectable within 24 h. The levels of FSH fell more slowly and were always detectable. In the second of the experiments, pulses of LH of normal amplitude were re-established in the immunized ewes by hourly intravenous injections of 200 ng of Des-Gly10 (Pro9)-LH-RH Ethylamide, a synthetic analogue of LH-RH that does not cross-react with the antiserum yet has similar biological activity to native LH-RH. The analogue was injected over 6 h and had no detectable effect on the levels of FSH. When the ewes were injected with 50 micrograms of oestradiol benzoate, the LH responses to the analogue were completely inhibited for 4-5 h then slowly recovered to normal levels. The oestrogen injection also depressed the levels of FSH for varying amounts of time. On the basis of the normal LH patterns and responses to oestrogen, we conclude that combining passive immunization against LH-RH with injections of an LH-RH analogue provides a valuable model for testing factors that affect pituitary responsiveness to LH-RH.
在两项针对去卵巢母羊的实验中，对促黄体激素释放激素（LH-RH）进行被动免疫后，测量了血浆中促黄体生成素（LH）和促卵泡生成素（FSH）的水平。LH的脉冲式分泌受到抑制，该激素水平迅速下降，24小时内无法检测到。FSH水平下降较慢，始终可检测到。在第二项实验中，通过每小时静脉注射200纳克去甘氨酸10（脯氨酸9）-LH-RH乙酰胺（一种LH-RH的合成类似物，与抗血清不发生交叉反应，但具有与天然LH-RH相似的生物活性），在免疫母羊中重新建立了正常幅度的LH脉冲。该类似物注射6小时，对FSH水平没有可检测到的影响。当给母羊注射50微克苯甲酸雌二醇时，LH对该类似物的反应在4至5小时内完全受到抑制，然后缓慢恢复到正常水平。雌激素注射也在不同时间段内降低了FSH水平。基于正常的LH模式和对雌激素的反应，我们得出结论，将针对LH-RH的被动免疫与LH-RH类似物注射相结合，为测试影响垂体对LH-RH反应性的因素提供了一个有价值的模型。
