Hoyes A D, Barber P
J Anat. 1976 Sep;122(Pt 1):113-20.
A study has been undertaken of the effects of a number of fixation procedures on the appearance of axons in the ureteric plexuses of the rat, and especially on the appearance of the dense-cored vesicles in those axons which have recently been characterized as pain afferents. Terminals were readily identified in material fixed by perfusion with glutaraldehyde, and many of them contained few if any vesicles. After fixation by immersion in cold glutaraldehyde terminals were not easy to identify because of the loss of microtubules. Immersion fixation in glutaraldehyde produced changes in the number and distribution of the vesicles in the axons which accentuated the similarity of the 'vesicle-containing regions' to the terminals of so-called 'purinergic' axons. Few of the dense-cored vesicles seen in glutaraldehyde-fixed material were preserved by fixation in permanganate, osmium or paraformaldehyde. The way dense-cored vesicles were or were not preserved by the various fixatives used indicated major differences between such cores and those of the vesicles in adrenergic axons. There was also evidence from the study of osmium-fixed material that both the number and appearance of the vesicles can be affected by the type of buffer used: the presence of more vesicles after fixation in osmium buffered with veronal than after osmium buffered in other ways was attributed to the membrane-stabilizing properties of veronal.
一项研究探讨了多种固定程序对大鼠输尿管丛中轴突外观的影响，尤其是对那些最近被确定为痛觉传入神经的轴突中致密核心小泡外观的影响。在用戊二醛灌注固定的材料中，终末很容易识别，而且其中许多终末几乎不含小泡。用冷戊二醛浸泡固定后，由于微管的丢失，终末不易识别。戊二醛浸泡固定使轴突中小泡的数量和分布发生变化，这加剧了“含小泡区域”与所谓“嘌呤能”轴突终末的相似性。在戊二醛固定材料中看到的致密核心小泡，很少能通过高锰酸盐、锇或多聚甲醛固定得以保存。所用各种固定剂对致密核心小泡的保存与否表明，这类小泡的核心与肾上腺素能轴突中小泡的核心存在重大差异。对锇固定材料的研究也有证据表明，小泡的数量和外观会受到所用缓冲液类型的影响：用巴比妥缓冲锇固定后比用其他方式缓冲锇固定后有更多小泡，这归因于巴比妥的膜稳定特性。
