Liggitt H D, Leid R W, Huston L
Vet Immunol Immunopathol. 1984 Aug;7(1):81-7. doi: 10.1016/0165-2427(84)90030-8.
Platelet activating factor is a multifaceted mediator of inflammation capable of stimulating platelet aggregation as well as anaphylaxis, neutropenia and numerous other in vitro and in vivo cellular changes. This lipid mediator, or autocoid, is released by a wide variety of inflammatory cells following an equally diverse group of cellular stimuli including phagocytosis or antigenic stimulation. The synthesized form of PAF is acetyl glyceryl ether phosphorylcholine (AGEPC). In this study AGEPC aggregated bovine platelets in a dose dependent manner. Maximal, irreversible aggregation occurred at 3.6 X 10(-11) M AGEPC with unwashed platelets and at 8.8 X 10(-12) M AGEPC with washed platelets. Aggregation failed to occur when platelets were tested with the biologically inactive structural analog of AGEPC. The possible contribution by platelet cyclooxygenase products was eliminated by showing lack of platelet aggregation to arachidonic acid and also by pretreating platelets with aspirin.
血小板活化因子是一种多方面的炎症介质，能够刺激血小板聚集以及过敏反应、嗜中性白血球减少症和许多其他体外和体内的细胞变化。这种脂质介质或自体活性物质，在包括吞噬作用或抗原刺激等同样多样的细胞刺激后，由多种炎症细胞释放。PAF的合成形式是乙酰甘油醚磷酸胆碱（AGEPC）。在本研究中，AGEPC以剂量依赖的方式使牛血小板聚集。对于未洗涤的血小板，在3.6×10（-11）M AGEPC时发生最大程度的不可逆聚集，对于洗涤过的血小板，在8.8×10（-12）M AGEPC时发生最大程度的不可逆聚集。当用AGEPC的无生物活性的结构类似物测试血小板时，未发生聚集。通过显示血小板对花生四烯酸无聚集反应以及用阿司匹林预处理血小板，排除了血小板环氧化酶产物的可能作用。
