Channing C P, Tanabe K, Chacon M, Tildon J T
Fertil Steril. 1984 Oct;42(4):598-605. doi: 10.1016/s0015-0282(16)48145-8.
Ovarian tissue obtained from three human infants (60, 120, and 210 days of age) was separated into cell types and cultured. Granulosa cells from two of three subjects were viable and grew in culture. The cells had the potential to secrete low levels of progesterone and responded to luteinizing hormone and follicle-stimulating hormone added in culture with greatly enhanced ability to secrete progesterone. Granulosa cells could also secrete inhibin activity in culture and responded to luteinizing hormone and follicle-stimulating hormone with enhanced inhibin secretion. The granulosa cells also had the potential to secrete estrogen in the presence of testosterone. Serum levels of gonadotropin in the human infant are elevated for a period between 1 and 4 months; yet only follicular growth, not luteinization, occurs. It can be concluded that infant human granulosa cells, like adult human granulosa cells, have the potential of responding in vitro to gonadotropin.
从三名人类婴儿（分别为60日龄、120日龄和210日龄）获取的卵巢组织被分离成不同细胞类型并进行培养。三名受试者中有两名的颗粒细胞存活并在培养中生长。这些细胞有分泌低水平孕酮的潜力，并且在培养中添加促黄体生成素和促卵泡激素后，分泌孕酮的能力会大大增强。颗粒细胞在培养中也能分泌抑制素活性，并且对促黄体生成素和促卵泡激素有反应，抑制素分泌增加。在有睾酮存在的情况下，颗粒细胞也有分泌雌激素的潜力。人类婴儿的促性腺激素血清水平在1至4个月期间会升高；然而，此时仅发生卵泡生长，而非黄体化。可以得出结论，婴儿人类颗粒细胞与成人人类颗粒细胞一样，有在体外对促性腺激素作出反应的潜力。
