Teranishi T, Hirano T, Lin B H, Onoue K
J Immunol. 1984 Dec;133(6):3062-7.
The effect of IL 2 on Staphylococcus aureus Cowan I (SAC)-driven IgG production of human B cells was examined by utilizing chromatographically purified IL 2 (R-IL 2) and the transcription product of the cloned cDNA for human IL 2 purified from recombinant E. coli (G-IL 2). Both preparations of IL 2 by themselves were not enough to induce optimal IgG-production in the SAC-stimulated tonsillar B cell fraction, which was highly enriched for B cells, but effectively induced IgG production in the presence of a subeffective number of T cells or a late-acting B cell differentiation factor (BCDF). In addition, the activity that induced IgG production in the presence of a subeffective number of T cells was absorbed with an IL 2-dependent mouse T cell line. These results clearly indicate that IL 2 has a definite effect on B cell differentiation in this system. Although the mechanisms of this effect remain to be elucidated, a direct effect of IL 2 on B cells may be involved, because the addition of IL 2 along with SAC induced a limited but significant increase of 3H-TdR incorporation in the highly enriched B cell population, which showed very little response to PHA and Con A even in the presence of IL 2, and, as mentioned above, IL 2 induced IgG production in the B cell preparation without any supplement of T cells provided the late-acting BCDF fraction was present in the culture.
通过使用色谱纯化的白细胞介素2（R-IL 2）和从重组大肠杆菌中纯化的人白细胞介素2克隆cDNA的转录产物（G-IL 2），研究了白细胞介素2对金黄色葡萄球菌考恩I株（SAC）驱动的人B细胞IgG产生的影响。单独的这两种白细胞介素2制剂都不足以在SAC刺激的扁桃体B细胞部分诱导最佳的IgG产生，该部分富含B细胞，但在亚有效数量的T细胞或晚期作用的B细胞分化因子（BCDF）存在下能有效诱导IgG产生。此外，在亚有效数量的T细胞存在下诱导IgG产生的活性被一种依赖白细胞介素2的小鼠T细胞系吸收。这些结果清楚地表明，在该系统中白细胞介素2对B细胞分化有明确作用。尽管这种作用的机制仍有待阐明，但白细胞介素2可能直接作用于B细胞，因为在高度富集的B细胞群体中，SAC与白细胞介素2一起添加会诱导3H-TdR掺入量有限但显著增加，但即使在有白细胞介素2存在的情况下，该群体对PHA和Con A的反应也非常小，并且如上所述，如果培养物中存在晚期作用的BCDF部分，白细胞介素2在不添加任何T细胞的B细胞制剂中可诱导IgG产生。
