一种用于筛选针对难溶性蛋白质的单克隆抗体的简单快速的固相酶联免疫吸附测定法。
A simple and rapid solid phase enzyme-linked immunoadsorbence assay for screening monoclonal antibodies to poorly soluble proteins.
作者信息
Evan G I
出版信息
J Immunol Methods. 1984 Oct 26;73(2):427-35. doi: 10.1016/0022-1759(84)90417-4.
PMID:6436385
Abstract
An ELISA is described in which protein antigens are fixed to microtitre wells. This assay is of particular use when antigens are soluble only in solutions containing SDS or other detergents, or else have been purified by preparative SDS polyacrylamide gel electrophoresis. Because the protein antigens are fixed to the microtitre wells, it is also possible to re-use antigen derivatised plates several times.
摘要
本文描述了一种酶联免疫吸附测定（ELISA），其中蛋白质抗原被固定在微量滴定孔中。当抗原仅溶于含有十二烷基硫酸钠（SDS）或其他去污剂的溶液中，或者已通过制备性SDS聚丙烯酰胺凝胶电泳纯化时，该测定法特别有用。由于蛋白质抗原被固定在微量滴定孔中，因此也可以多次重复使用衍生化抗原的平板。
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