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A rapid and simple method for efficient coating of microtiter plates using low amounts of antigen in the presence of detergent.

作者信息

Drexler G, Eichinger A, Wolf C, Sieghart W

出版信息

J Immunol Methods. 1986 Dec 4;95(1):117-22. doi: 10.1016/0022-1759(86)90325-x.

DOI:10.1016/0022-1759(86)90325-x
PMID:3023494
Abstract

Bio-Beads SM-2 have previously been used for the removal of non-ionic detergents from protein solutions. Addition of Bio-Beads SM-2 to detergent solubilized antigen significantly enhanced the immobilization of antigen to microtiter wells. Depending on the incubation time used 35-45% of the applied antigen could be immobilized to the microtiter wells. Using this method and a subsequent ELISA procedure it was possible to detect monoclonal antibodies in hybridoma supernatants after coating microtiter wells with 100 microliters of a solution containing 16 ng antigen/ml in the presence of 0.01% Triton X-100.

摘要

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