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分离出一个与一种34千道尔顿蛋白质（它是pp60v-src的底物）的序列互补的cDNA克隆。

Isolation of a cDNA clone complementary to sequences for a 34-kilodalton protein which is a pp60v-src substrate.

作者信息

Tomasiewicz H G, Cook-Deegan R, Chikaraishi D M

出版信息

Mol Cell Biol. 1984 Sep;4(9):1935-8. doi: 10.1128/mcb.4.9.1935-1938.1984.

DOI:10.1128/mcb.4.9.1935-1938.1984
PMID:6436690
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC369003/
Abstract

We have isolated a partial cDNA clone containing sequences complementary to a mRNA encoding a 34- to 36-kilodalton normal chicken cell protein which is a substrate for pp60v-src kinase activity. Using this 34-kilodalton cDNA clone as a probe, we determined that the size of the 34-kilodalton mRNA was 1,100 nucleotides and the level of the 34-kilodalton RNA was the same in various tissues of mature chickens but was significantly higher in chicken embryo fibroblast cells.

摘要

我们分离出了一个部分cDNA克隆，其包含与编码一种34至36千道尔顿正常鸡细胞蛋白的mRNA互补的序列，该蛋白是pp60v-src激酶活性的底物。使用这个34千道尔顿的cDNA克隆作为探针，我们确定34千道尔顿mRNA的大小为1100个核苷酸，并且在成熟鸡的各种组织中34千道尔顿RNA的水平相同，但在鸡胚成纤维细胞中显著更高。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7a7/369003/63fef5c8f8ce/molcellb00151-0275-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7a7/369003/741f942cb5f6/molcellb00151-0274-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7a7/369003/5f3f06642c3d/molcellb00151-0274-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7a7/369003/63fef5c8f8ce/molcellb00151-0275-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7a7/369003/741f942cb5f6/molcellb00151-0274-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7a7/369003/5f3f06642c3d/molcellb00151-0274-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7a7/369003/63fef5c8f8ce/molcellb00151-0275-a.jpg

相似文献

1
Isolation of a cDNA clone complementary to sequences for a 34-kilodalton protein which is a pp60v-src substrate.分离出一个与一种34千道尔顿蛋白质（它是pp60v-src的底物）的序列互补的cDNA克隆。
Mol Cell Biol. 1984 Sep;4(9):1935-8. doi: 10.1128/mcb.4.9.1935-1938.1984.
2
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Mol Cell Biol. 1993 Dec;13(12):7892-900. doi: 10.1128/mcb.13.12.7892-7900.1993.

本文引用的文献

1
Tissue-specific expression of an unusual H-2 (class I)-related gene.一个不寻常的与H-2（I类）相关基因的组织特异性表达。
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4947-51. doi: 10.1073/pnas.79.16.4947.
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Identification and characterization of cellular targets for tyrosine protein kinases.酪氨酸蛋白激酶细胞靶点的鉴定与表征
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Subcellular location of an abundant substrate (p36) for tyrosine-specific protein kinases.酪氨酸特异性蛋白激酶的一种丰富底物（p36）的亚细胞定位。
Mol Cell Biol. 1983 Mar;3(3):340-50. doi: 10.1128/mcb.3.3.340-350.1983.
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Biochemical characterization of a 34-kilodalton normal cellular substrate of pp60v-src and an associated 6-kilodalton protein.pp60v-src的一种34千道尔顿正常细胞底物及一种相关的6千道尔顿蛋白质的生化特性
Mol Cell Biol. 1984 Jan;4(1):77-85. doi: 10.1128/mcb.4.1.77-85.1984.
5
The 34 kd pp60src substrate is located at the inner face of the plasma membrane.34千道尔顿的pp60src底物位于质膜的内表面。
Cell. 1983 Jul;33(3):767-79. doi: 10.1016/0092-8674(83)90019-3.
6
Comparison of the 34,000-Da pp60src substrate and a 38,000-Da phosphoprotein identified by monoclonal antibodies.由单克隆抗体鉴定的34000道尔顿的pp60src底物与一种38000道尔顿磷蛋白的比较。
J Biol Chem. 1983 Jul 10;258(13):8497-502.
7
Association of pp36, a phosphorylated form of the presumed target protein for the src protein of Rous sarcoma virus, with the membrane of chicken cells transformed by Rous sarcoma virus.pp36（劳斯肉瘤病毒src蛋白假定靶蛋白的磷酸化形式）与经劳斯肉瘤病毒转化的鸡细胞的膜的关联。
Proc Natl Acad Sci U S A. 1983 Feb;80(4):960-4. doi: 10.1073/pnas.80.4.960.
8
High-efficiency cloning of full-length cDNA.全长cDNA的高效克隆
Mol Cell Biol. 1982 Feb;2(2):161-70. doi: 10.1128/mcb.2.2.161-170.1982.
9
Phosphorylation of a 36,000 Mr cellular protein in cells infected with partial transformation mutants of rous sarcoma virus.在感染劳氏肉瘤病毒部分转化突变体的细胞中一种分子量为36,000的细胞蛋白的磷酸化作用
Mol Cell Biol. 1982 Feb;2(2):147-53. doi: 10.1128/mcb.2.2.147-153.1982.
10
Cytosolic malic dehydrogenase activity is associated with a putative substrate for the transforming gene product of Rous sarcoma virus.胞质苹果酸脱氢酶活性与劳氏肉瘤病毒转化基因产物的一种假定底物相关。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):228-32. doi: 10.1073/pnas.79.2.228.