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Isolation and characterization of ras-transfected BALB/3T3 clone showing morphological transformation by 12-O-tetradecanoyl-phorbol-13-acetate.通过12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯显示形态转化的ras转染BALB / 3T3克隆的分离与鉴定。
Jpn J Cancer Res. 1988 Aug;79(8):921-30. doi: 10.1111/j.1349-7006.1988.tb00056.x.
2
Tumor-promoting phorbol ester and ras oncogene expression inhibit the glucocorticoid-dependent transcription from the mouse mammary tumor virus long terminal repeat.促肿瘤佛波酯和ras癌基因的表达抑制了来自小鼠乳腺肿瘤病毒长末端重复序列的糖皮质激素依赖性转录。
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Detection of initiating as well as promoting activity of chemicals by a novel cell transformation assay using v-Ha-ras-transfected BALB/c 3T3 cells (Bhas 42 cells).通过使用v-Ha-ras转染的BALB/c 3T3细胞（Bhas 42细胞）的新型细胞转化试验检测化学物质的启动和促进活性。
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Mol Cell Biol. 1990 Aug;10(8):4155-62. doi: 10.1128/mcb.10.8.4155-4162.1990.

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Flat reversion by okadaic acid of raf and ret-II transformants.冈田酸对raf和ret-II转化体的扁平回复
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Cell variants showing differential susceptibility to ultraviolet light--induced transformation.对紫外线诱导转化表现出不同敏感性的细胞变体。
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Parameters governing the transfer of the genes for thymidine kinase and dihydrofolate reductase into mouse cells using metaphase chromosomes or DNA.使用中期染色体或DNA将胸苷激酶和二氢叶酸还原酶基因转移到小鼠细胞中的调控参数。
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Enhancement of chemical-carcinogen-induced cell transformation in hamster embryo cells by 1 alpha,25-dihydroxycholecalciferol, the biologically active metabolite of vitamin D3.维生素D3的生物活性代谢产物1α,25 - 二羟胆钙化醇增强化学致癌物诱导的仓鼠胚胎细胞转化
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通过12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯显示形态转化的ras转染BALB / 3T3克隆的分离与鉴定。

Isolation and characterization of ras-transfected BALB/3T3 clone showing morphological transformation by 12-O-tetradecanoyl-phorbol-13-acetate.

作者信息

Sasaki K, Mizusawa H, Ishidate M

机构信息

Laboratory of Cell Development, National Institute of Hygienic Sciences, Tokyo.

出版信息

Jpn J Cancer Res. 1988 Aug;79(8):921-30. doi: 10.1111/j.1349-7006.1988.tb00056.x.

DOI:10.1111/j.1349-7006.1988.tb00056.x
PMID:3141328
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5917616/
Abstract

The transformation frequency of mouse BALB/3T3 cells was significantly enhanced after transfection with an activated ras oncogene (v-Ha-ras) followed by treatment with a tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), suggesting that the ras oncogene acted as an initiator in two-stage carcinogenesis. A cell clone (Bhas42) containing the ras oncogene was isolated from the ras-transfected BALB/3T3 cells. Bhas42 cells were flat and showed contact inhibition, but the addition of TPA to quiescent Bhas42 cultures resulted in a dramatic change of cell morphology to spindle shape, doubling of the cell population, and increased DNA synthesis.

摘要

用活化的ras癌基因（v-Ha-ras）转染小鼠BALB/3T3细胞，随后用肿瘤促进剂12-O-十四烷酰佛波醇-13-乙酸酯（TPA）处理后，其转化频率显著提高，这表明ras癌基因在两阶段致癌过程中起启动子的作用。从经ras转染的BALB/3T3细胞中分离出一个含有ras癌基因的细胞克隆（Bhas42）。Bhas42细胞扁平，表现出接触抑制，但向静止的Bhas42培养物中添加TPA会导致细胞形态急剧变为纺锤形，细胞数量加倍，并增加DNA合成。