通过12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯显示形态转化的ras转染BALB / 3T3克隆的分离与鉴定。
Isolation and characterization of ras-transfected BALB/3T3 clone showing morphological transformation by 12-O-tetradecanoyl-phorbol-13-acetate.
作者信息
Sasaki K, Mizusawa H, Ishidate M
机构信息
Laboratory of Cell Development, National Institute of Hygienic Sciences, Tokyo.
出版信息
Jpn J Cancer Res. 1988 Aug;79(8):921-30. doi: 10.1111/j.1349-7006.1988.tb00056.x.
Abstract
The transformation frequency of mouse BALB/3T3 cells was significantly enhanced after transfection with an activated ras oncogene (v-Ha-ras) followed by treatment with a tumor promoter, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), suggesting that the ras oncogene acted as an initiator in two-stage carcinogenesis. A cell clone (Bhas42) containing the ras oncogene was isolated from the ras-transfected BALB/3T3 cells. Bhas42 cells were flat and showed contact inhibition, but the addition of TPA to quiescent Bhas42 cultures resulted in a dramatic change of cell morphology to spindle shape, doubling of the cell population, and increased DNA synthesis.
摘要
用活化的ras癌基因（v-Ha-ras）转染小鼠BALB/3T3细胞，随后用肿瘤促进剂12-O-十四烷酰佛波醇-13-乙酸酯（TPA）处理后，其转化频率显著提高，这表明ras癌基因在两阶段致癌过程中起启动子的作用。从经ras转染的BALB/3T3细胞中分离出一个含有ras癌基因的细胞克隆（Bhas42）。Bhas42细胞扁平，表现出接触抑制，但向静止的Bhas42培养物中添加TPA会导致细胞形态急剧变为纺锤形，细胞数量加倍，并增加DNA合成。
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