Farrugia A, Griffin B, Pepper D, Prowse C
Thromb Haemost. 1984 Jul 29;51(3):338-42.
Early work on the purification of factor VIII using polyethylene glycol (PEG) indicated that other polymers might also be used to precipitate factor VIII leaving fibrinogen in solution. Recently polyvinylpyrrolidone (PVP) has also been advocated for this purpose. In this study, different concentrations and molecular weights of hydroxyethyl starch, dextran, PEG, PVP, Ficoll, Percoll and albumin were examined for their ability to precipitate the factor VIII complex from cooled (not frozen) fresh CPD plasma. At optimal concentrations near quantitative recovery of VIIIR:Ag and VIII:CAg was achieved in 2 hr with minimal precipitation of fibrinogen or total protein. The best separations were observed with hydroxyethyl starch, albumin or Ficoll. PVP and PEG gave inferior purifications. Results for cryoprecipitate and intermediate-purity factor VIII concentrate were inferior to those obtained with plasma. Simple pre-concentration of plasma prior to chilling is an attractive alternative for large scale continuous production.
早期使用聚乙二醇（PEG）纯化因子VIII的工作表明，其他聚合物也可用于沉淀因子VIII，而使纤维蛋白原留在溶液中。最近，聚乙烯吡咯烷酮（PVP）也被提倡用于此目的。在本研究中，检测了不同浓度和分子量的羟乙基淀粉、右旋糖酐、PEG、PVP、菲可、 Percoll和白蛋白从冷却（未冷冻）的新鲜CPD血浆中沉淀因子VIII复合物的能力。在最佳浓度下，2小时内可实现VIIIR:Ag和VIII:CAg的近乎定量回收，同时纤维蛋白原或总蛋白的沉淀最少。使用羟乙基淀粉、白蛋白或菲可观察到最佳分离效果。PVP和PEG的纯化效果较差。冷沉淀和中等纯度因子VIII浓缩物的结果不如从血浆中获得的结果。在冷却前对血浆进行简单预浓缩是大规模连续生产的一个有吸引力的替代方法。
