Casillas G, Simonetti C
Br J Haematol. 1982 Apr;50(4):665-72. doi: 10.1111/j.1365-2141.1982.tb01967.x.
The PVP precipitating properties for human and bovine factor VIII and fibrinogen were studied and a new technique for the fractionation of factor VIII and fibrinogen was developed. The precipitation was performed at different temperatures and different PVP concentrations and the best conditions for the technique were chosen. The technique consists of two steps: (a) precipitation of factor VIII from undiluted plasma with PVP and (b) washing of the precipitate with a glycine--saline solution. The final concentrate contains 90% of the factor VIII and 20% of the fibrinogen of the original plasma.
研究了聚乙烯吡咯烷酮（PVP）对人及牛凝血因子VIII和纤维蛋白原的沉淀特性，并开发了一种分离凝血因子VIII和纤维蛋白原的新技术。在不同温度和不同PVP浓度下进行沉淀，并选择了该技术的最佳条件。该技术包括两个步骤：（a）用PVP从未稀释的血浆中沉淀凝血因子VIII；（b）用甘氨酸 - 盐溶液洗涤沉淀物。最终浓缩物含有原始血浆中90%的凝血因子VIII和20%的纤维蛋白原。
