De Fazio S R, Monaco A P, Gozzo J J
Diagn Immunol. 1983;1(4):276-83.
Because urinary C-reactive protein (CRP) has potential as a diagnostic marker, a sensitive enzyme immunoassay has been developed to measure this substance. This assay involves immobilizing antibody to CRP on a plastic surface, followed sequentially by addition of sample to be assayed, anti-CRP labeled with biotin, avidin conjugated with enzyme, and substrate. As little as 0.5 ng of CRP can be detected by the assay, which is reproducible and correlates well with a radial immunodiffusion method. Because the assay is inhibited by urine samples having a low pH or high urea concentration, it is necessary to pretreat the samples by solvent exchange in a commercial, disposable concentration unit. Application of the assay to clinical specimens gives results similar to those obtained previously by a complement fixation assay.
由于尿C反应蛋白（CRP）具有作为诊断标志物的潜力，因此已开发出一种灵敏的酶免疫测定法来检测该物质。该测定法包括将抗CRP抗体固定在塑料表面，然后依次加入待检测样品、生物素标记的抗CRP、与酶结合的抗生物素蛋白和底物。该测定法可检测低至0.5 ng的CRP，具有可重复性，且与放射免疫扩散法相关性良好。由于该测定法会受到低pH值或高尿素浓度尿液样本的抑制，因此有必要在商用一次性浓缩装置中通过溶剂交换对样本进行预处理。将该测定法应用于临床标本，所得结果与先前通过补体结合试验获得的结果相似。
