Coll J M
Instituto Nacional de Investigaciones Agrarias, Madrid, Spain.
Rev Esp Fisiol. 1988 Dec;44(4):359-67.
A fast sandwich enzyme immunoassay has been developed for C-reactive protein (CRP). This method can be used for screening CRP concentration in large numbers of samples providing a non precipitation, non agglutination and non radioactive alternative for assessment of human CRP. Advantages over previously reported CRP sandwich assays include: assay time was reduced from 4 1/2 h to 45 min, incubations were made at room temperature instead of 37 degrees C and serum dilutions required were 100-400 fold instead of 10000-20000 fold. Correlations were good with both nephelometry and phosphorylethanolamine binding assay. The 45% false positives found with the slide-latex anti C-reactive protein method were reduced to 0% by the use of the described method.
已开发出一种用于检测C反应蛋白（CRP）的快速夹心酶免疫测定法。该方法可用于大量样本中CRP浓度的筛查，为评估人体CRP提供了一种非沉淀、非凝集且非放射性的替代方法。与先前报道的CRP夹心测定法相比，其优势包括：测定时间从4.5小时缩短至45分钟，孵育在室温而非37℃下进行，所需的血清稀释倍数为100 - 400倍而非10000 - 20000倍。与散射比浊法和磷酸乙醇胺结合测定法的相关性良好。使用所述方法可将玻片 - 乳胶抗C反应蛋白法中发现的45%的假阳性率降至0%。
