Aucouturier P, Preud'homme J L, Lubochinsky B
Diagn Immunol. 1983;1(4):310-4.
Fluorescence polarization immunoassay (FPIA) offers a good alternative to isotopic methods for the determination of drug and hormone levels in biological fluids. We applied it to estradiol, using a fluoresceinated derivative of estradiol and the IgG fraction of a highly specific rabbit antiserum. After determining the optimal operational conditions, FPIA was used for measuring urinary concentrations of free estradiol in normal and pregnant women, after a purification step on a reversed phase cartridge. The method turned out to be rapid (1 min to reach the equilibrium state) and accurate.
荧光偏振免疫分析法（FPIA）为测定生物体液中的药物和激素水平提供了一种优于同位素方法的良好替代方法。我们将其应用于雌二醇，使用雌二醇的荧光衍生物和高特异性兔抗血清的IgG组分。在确定最佳操作条件后，FPIA用于测量正常和孕妇尿液中游离雌二醇的浓度，这是在反相柱上进行纯化步骤之后。结果表明该方法快速（1分钟达到平衡状态）且准确。
