Dandliker W B, Hicks A N, Levison S A, Brawn R J
Res Commun Chem Pathol Pharmacol. 1977 Sep;18(1):147-56.
A fluorescent estradiol derivative binds strongly to antiestradiol antibody. The binding, measured by fluorescence polarization, is inhibited by estradiol and by diethylstilbestrol. Tentatively characterized as N-(estradiol-6-iminooxyacetyl) fluorescein amine, the derivative was prepared from estradiol-6-iminooxyacetic acid, dicyclohexylcarbodiimide, and fluorescein amine, and isolated by TLC. It has a fluorescence emission similar to that of fluorescein and an absorption spectrum consistent with a fluorescein: estradiol molar ratio of 1:1.
一种荧光雌二醇衍生物与抗雌二醇抗体紧密结合。通过荧光偏振测量的这种结合，会受到雌二醇和己烯雌酚的抑制。该衍生物初步鉴定为N-(雌二醇-6-亚氨基氧基乙酰基)荧光素胺，它由雌二醇-6-亚氨基氧基乙酸、二环己基碳二亚胺和荧光素胺制备而成，并通过薄层色谱法分离。它的荧光发射与荧光素相似，吸收光谱与荧光素：雌二醇摩尔比为1:1一致。
