Podell S B, Vacquier V D
Exp Cell Res. 1984 Dec;155(2):467-76. doi: 10.1016/0014-4827(84)90207-6.
The egg jelly-induced acrosome reaction is inhibited by polyclonal antibodies raised against either of two S. purpuratus sperm-membrane proteins, of Mr 80 and 210 kD. Although the two antigens used have dissimilar CNBr peptide maps, antisera produced against each of them cross-react with both proteins. Inhibition of the egg jelly-induced acrosome reaction by the antisera is bypassed by a combination of the ionophores monensin and A23187. This result, along with data showing that the antisera inhibit egg jelly-induced uptake of 45Ca2+, suggests that the antisera may block both Ca2+ uptake and Na+/H+ exchange in the sperm. The acrosome reaction blockage appears to be caused by the same component of the polyclonal sera responsible for cross-reaction; consequently, these antisera cannot be used to determine whether one or both of the cross-reacting proteins modulate a critical step in the acrosome reaction.
用针对两种海胆精子膜蛋白（分子量分别为80kD和210kD）之一制备的多克隆抗体可抑制卵胶诱导的顶体反应。尽管所使用的两种抗原具有不同的溴化氰肽图谱，但针对它们各自产生的抗血清与这两种蛋白都发生交叉反应。离子载体莫能菌素和A23187的组合可绕过抗血清对卵胶诱导的顶体反应的抑制作用。这一结果，连同表明抗血清抑制卵胶诱导的45Ca2+摄取的数据，提示抗血清可能会阻断精子中Ca2+的摄取和Na+/H+交换。顶体反应的阻断似乎是由多克隆血清中负责交叉反应的同一成分引起的；因此，这些抗血清不能用于确定交叉反应的蛋白中的一种还是两种调节了顶体反应中的关键步骤。
