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1
Wheat germ agglutinin blocks the acrosome reaction in Strongylocentrotus purpuratus sperm by binding a 210,000-mol-wt membrane protein.小麦胚凝集素通过结合一种分子量为210,000的膜蛋白来阻断紫海胆精子的顶体反应。
J Cell Biol. 1984 Nov;99(5):1598-604. doi: 10.1083/jcb.99.5.1598.
2
Inhibition of sea urchin sperm acrosome reaction by antibodies directed against two sperm membrane proteins. Characterization and mechanism of action.针对两种精子膜蛋白的抗体对海胆精子顶体反应的抑制作用。特性及作用机制。
Exp Cell Res. 1984 Dec;155(2):467-76. doi: 10.1016/0014-4827(84)90207-6.
3
Internal pH can regulate Ca2+ uptake and the acrosome reaction in sea urchin sperm.细胞内pH值可调节海胆精子对钙离子的摄取及顶体反应。
Dev Biol. 1987 Mar;120(1):112-20. doi: 10.1016/0012-1606(87)90109-6.
4
Reduction of the fertilizing capacity of sea urchin sperm by cannabinoids derived from marihuana. I. Inhibition of the acrosome reaction induced by egg jelly.大麻衍生的大麻素降低海胆精子的受精能力。I. 抑制卵胶诱导的顶体反应。
Mol Reprod Dev. 1991 May;29(1):51-9. doi: 10.1002/mrd.1080290109.
5
Localization of wheat germ agglutinin and antibody binding sites on the plasma membranes of sea urchin sperm heads as revealed by label-fracture and fracture-flip.通过标记断裂和断裂翻转揭示的小麦胚凝集素和抗体结合位点在海胆精子头部质膜上的定位
Mol Reprod Dev. 1991 Apr;28(4):410-8. doi: 10.1002/mrd.1080280414.
6
Induction of acrosomal reaction and calcium uptake in ram spermatozoa by ionophores.离子载体诱导公羊精子顶体反应和钙摄取
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7
Involvement of Wheat Germ Agglutinin (WGA)-Binding Protein in the Induction of the Acrosome Reaction of the Sea Urchin, Strongylocentrotus intermedius. I. WGA Affects the Ion Fluxes Associated with the Acrosome Reaction: (sea urchin/acrosome reaction/wheat germ agglutinin/ion flux/species specificity).
Dev Growth Differ. 1989 Oct;31(5):459-466. doi: 10.1111/j.1440-169X.1989.00459.x.
8
Phosphorylation of sperm histone H1 is induced by the egg jelly layer in the sea urchin Strongylocentrotus purpuratus.紫海胆卵胶层可诱导精子组蛋白H1发生磷酸化。
Dev Biol. 1986 Jul;116(1):203-12. doi: 10.1016/0012-1606(86)90057-6.
9
Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca2+ and H+ fluxes of sea urchin sperm.针对一种膜糖蛋白的单克隆抗体可抑制海胆精子的顶体反应以及相关的钙离子和氢离子通量。
Cell. 1985 Mar;40(3):697-703. doi: 10.1016/0092-8674(85)90218-1.
10
Pretreatment effects of jelly components on the sperm acrosome reaction and histone degradation in the starfish, Asterina pectinifera.
Biochem Biophys Res Commun. 1992 Aug 31;187(1):268-73. doi: 10.1016/s0006-291x(05)81487-1.

引用本文的文献

1
The sea urchin sperm receptor for egg jelly is a modular protein with extensive homology to the human polycystic kidney disease protein, PKD1.海胆卵胶膜的精子受体是一种模块化蛋白质，与人类多囊肾病蛋白PKD1具有广泛的同源性。
J Cell Biol. 1996 May;133(4):809-17. doi: 10.1083/jcb.133.4.809.
2
Monoclonal antibodies to a membrane glycoprotein induce the phosphorylation of histone H1 in sea urchin spermatozoa.针对一种膜糖蛋白的单克隆抗体可诱导海胆精子中组蛋白H1的磷酸化。
J Cell Biol. 1988 Dec;107(6 Pt 1):2021-7. doi: 10.1083/jcb.107.6.2021.
3
Polarization of plasma membrane glycoconjugates in amphibian epidermis during metamorphosis.
Histochemistry. 1989;90(6):453-8. doi: 10.1007/BF00494356.
4
Lectin-binding pattern on the surface epidermis of Ambystoma tigrinum larvae. A light- and electron-microscopic study.虎纹钝口螈幼虫表皮表面的凝集素结合模式。一项光镜和电镜研究。
Histochemistry. 1987;87(5):431-8. doi: 10.1007/BF00496814.
5
Light- and electron-microscopic immunocytochemistry and lectin histochemistry of the subcommissural organ: evidence for processing of the secretory material.连合下器官的光镜和电镜免疫细胞化学及凝集素组织化学：分泌物质加工的证据
Cell Tissue Res. 1986;243(3):545-59. doi: 10.1007/BF00218061.
6
Secretory glycoproteins of the rat subcommissural organ are N-linked complex-type glycoproteins. Demonstration by combined use of lectins and specific glycosidases, and by the administration of Tunicamycin.大鼠连合下器官的分泌性糖蛋白是N-连接的复合型糖蛋白。通过凝集素和特定糖苷酶的联合使用以及衣霉素给药进行证明。
Histochemistry. 1990;93(6):607-15. doi: 10.1007/BF00272203.

本文引用的文献

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A partial sequence of ionic changes associated with the acrosome reaction of Strongylocentrotus purpuratus.与紫海胆顶体反应相关的离子变化的部分序列。
Dev Biol. 1981 Jan 15;81(1):145-54. doi: 10.1016/0012-1606(81)90357-2.
2
Jelly coat and induction of the acrosome reaction in echinoid sperm.海胆精子的透明带及顶体反应的诱导
Dev Biol. 1981 Aug;86(1):87-93. doi: 10.1016/0012-1606(81)90318-3.
3
Location of the N-acetyl-D-neuraminic acid binding site in wheat germ agglutinin. A crystallographic study at 2.8 A resolution.
J Mol Biol. 1980 May 5;139(1):53-60. doi: 10.1016/0022-2836(80)90115-1.
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Isolation of an arbacia sperm fertilization antigen.一种海胆精子受精抗原的分离。
J Exp Zool. 1982 May 20;221(1):101-5. doi: 10.1002/jez.1402210113.
5
Antibody to a sperm surface glycoprotein inhibits the egg jelly-induced acrosome reaction of sea urchin sperm.针对精子表面糖蛋白的抗体可抑制海胆精子的卵胶诱导的顶体反应。
Dev Biol. 1980 Oct;79(2):325-33. doi: 10.1016/0012-1606(80)90119-0.
6
Membrane potential depolarization and increased intracellular pH accompany the acrosome reaction of sea urchin sperm.膜电位去极化和细胞内pH值升高伴随着海胆精子的顶体反应。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6066-70. doi: 10.1073/pnas.78.10.6066.
7
Purification and use of limulin: a sialic acid-specific lectin.鲎试剂的纯化与应用：一种唾液酸特异性凝集素
J Histochem Cytochem. 1982 Sep;30(9):938-46. doi: 10.1177/30.9.6897073.
8
Mechanism of univalent antisperm antibody inhibition of fertilization in the sea urchin, Arbacia punctulata.单克隆抗精子抗体对海胆（马粪海胆）受精的抑制机制
J Exp Zool. 1982 May 20;221(1):93-9. doi: 10.1002/jez.1402210112.
9
Changes in internal pH associated with initiation of motility and acrosome reaction of sea urchin sperm.与海胆精子运动起始和顶体反应相关的细胞内pH变化。
Dev Biol. 1983 Jan;95(1):31-45. doi: 10.1016/0012-1606(83)90004-0.
10
endo-beta-N-acetylglucosaminidase F: endoglycosidase from Flavobacterium meningosepticum that cleaves both high-mannose and complex glycoproteins.内切-β-N-乙酰氨基葡萄糖苷酶F：来自脑膜败血黄杆菌的内切糖苷酶，可切割高甘露糖型和复合型糖蛋白。
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4540-4. doi: 10.1073/pnas.79.15.4540.

小麦胚凝集素通过结合一种分子量为210,000的膜蛋白来阻断紫海胆精子的顶体反应。

Wheat germ agglutinin blocks the acrosome reaction in Strongylocentrotus purpuratus sperm by binding a 210,000-mol-wt membrane protein.

作者信息

Podell S B, Vacquier V D

出版信息

J Cell Biol. 1984 Nov;99(5):1598-604. doi: 10.1083/jcb.99.5.1598.

DOI:10.1083/jcb.99.5.1598
PMID:6092388
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2113364/
Abstract

Wheat germ agglutinin (WGA) binds to the entire surface of Strongylocentrotus purpuratus sperm, and inhibits the egg jelly-induced acrosome reaction. The binding was found to be species dependent and was completely inhibited by 5 mM N-acetyl-D-glucosamine. Blockage of the acrosome reaction by WGA was bypassed by a combination of the ionophores A23187 and monensin, although neither ionophore was effective individually. These experiments suggest that WGA blocks both Ca2+ uptake and Na+/H+ exchange in these sperm, which was confirmed by direct measurements of 45Ca2+ uptake and H+ efflux. The target of WGA in S. purpuratus sperm appears to be a membrane glycoprotein of Mr = 210,000. Treatment of this protein with neuraminidase or endo-beta-N-acetylglucosaminidase F abolished WGA binding.

摘要

小麦胚凝集素（WGA）能结合到紫海胆精子的整个表面，并抑制卵胶诱导的顶体反应。研究发现这种结合具有物种依赖性，且能被5 mM的N-乙酰-D-葡萄糖胺完全抑制。尽管单独使用离子载体A23187和莫能菌素都无效，但两者联合使用可绕过WGA对顶体反应的阻断作用。这些实验表明，WGA可阻断这些精子中的Ca2+摄取和Na+/H+交换，这一点通过对45Ca2+摄取和H+外流的直接测量得到了证实。WGA在紫海胆精子中的作用靶点似乎是一种分子量为210,000的膜糖蛋白。用神经氨酸酶或内切-β-N-乙酰氨基葡萄糖苷酶F处理该蛋白会消除WGA的结合。