Mok W Y, Carvalho C M
J Med Microbiol. 1984 Dec;18(3):327-33. doi: 10.1099/00222615-18-3-327.
In a survey of 234 Amazonian toads and frogs, six strains of Mycobacterium chelonei subsp. abscessus were isolated from the liver or spleen of four of 66 Bufo marinus (6.1%) and from the kidney or peritoneal fluid of two of 86 B. granulosus (2.3%). There were no histopathological lesions in the viscera of the infected animals. Experimental infection of 29 captive B. marinus and B. granulosus, by the intraperitoneal route, with a pooled inoculum of M. chelonei subsp. abscessus caused five deaths near the end of a 2-month observation period. M. chelonei subsp. abscessus was isolated from the liver, spleen, kidney, gonad, heart and lung of toads killed at various intervals after inoculation, and intracellular acid-fast bacilli were seen in these organs. Histological evidence of invasion of tissues by mycobacteria became apparent from the 45th day after infection. The susceptibility to infection of B. marinus and B. granulosus suggests that these toads may serve as a fortuitous animal host for M. chelonei subsp. abscessus.
在一项对234只亚马逊蟾蜍和青蛙的调查中，从66只海蟾蜍（Bufo marinus）中的4只（6.1%）的肝脏或脾脏以及86只粒疣蟾蜍（B. granulosus）中的2只（2.3%）的肾脏或腹腔液中分离出了6株龟分枝杆菌脓肿亚种。受感染动物的内脏没有组织病理学损伤。通过腹腔途径，用龟分枝杆菌脓肿亚种的混合接种物对29只圈养的海蟾蜍和粒疣蟾蜍进行实验性感染，在2个月的观察期接近尾声时导致5只死亡。在接种后不同时间处死的蟾蜍的肝脏、脾脏、肾脏、性腺、心脏和肺中分离出了龟分枝杆菌脓肿亚种，并且在这些器官中发现了细胞内抗酸杆菌。感染后第45天开始出现分枝杆菌侵袭组织的组织学证据。海蟾蜍和粒疣蟾蜍对感染的易感性表明，这些蟾蜍可能是龟分枝杆菌脓肿亚种的偶然动物宿主。
