Hicks D R, Kraml M
Ther Drug Monit. 1984;6(3):328-33. doi: 10.1097/00007691-198409000-00013.
An ultraviolet spectrophotometric method and a high-performance liquid chromatographic (HPLC) method were developed for the determination of tolrestat, a novel aldose reductase inhibitor, in serum. The limits of detection of the methods are 15 micrograms/ml and 0.2 microgram/ml, respectively. With human serum, a modification of the HPLC method provides sensitivity to 25 ng/ml. The specificity of the methods were compared. The HPLC method can be applied to the lens and the sciatic nerve.
建立了紫外分光光度法和高效液相色谱（HPLC）法用于测定血清中新型醛糖还原酶抑制剂托瑞司他。这两种方法的检测限分别为15微克/毫升和0.2微克/毫升。对于人血清，对HPLC法进行改进后可检测至25纳克/毫升。比较了这两种方法的特异性。HPLC法可应用于晶状体和坐骨神经。
