Murrah V, Crosson J, Sauk J
Virchows Arch A Pathol Anat Histopathol. 1984;405(1):141-54. doi: 10.1007/BF00694932.
Direct immunofluorescence employing goat anti-human IgG, IgA, IgM, C3 component of complement, fibrinogen, albumin, and polyvalent immunoglobulins was performed on postmortem samples of gingiva, parotid gland, thyroid, kidney, and pancreas tissue of 15 diabetic and 15 control patients. Basement membrane thickness quantification of kidney tubules, gingival capillaries, and parotid gland ducts and acini was also done utilizing a calibrated magnifier on uniformly enlarged photomicrographs which had been specially stained to highlight basement membranes. Results revealed binding of IgG, albumin, and polyvalent immunoglobulin to kidney glomerular and tubular basement membranes and parotid ductal and acinar basement membranes in all diabetic subjects. Thyroid follicular basement membranes were positive in 8 of 15 diabetic patients for the same antisera. All gingival and pancreatic tissue from diabetic and control patients was negative for binding of all serum proteins tested. Basement membrane thickening in kidney tubules and gingival capillaries was observed in diabetic subjects; however, there was no apparent difference between diabetic and control patients in thickness of ductal or acinar basement membranes of the parotid gland.
对15名糖尿病患者和15名对照患者的牙龈、腮腺、甲状腺、肾脏和胰腺组织的尸检样本进行了直接免疫荧光检测，检测试剂包括山羊抗人IgG、IgA、IgM、补体C3成分、纤维蛋白原、白蛋白和多价免疫球蛋白。利用校准放大镜，在经过特殊染色以突出基底膜的均匀放大的显微照片上，对肾小管、牙龈毛细血管以及腮腺导管和腺泡的基底膜厚度进行了量化。结果显示，在所有糖尿病受试者中，IgG、白蛋白和多价免疫球蛋白与肾小球和肾小管基底膜以及腮腺导管和腺泡基底膜结合。在15名糖尿病患者中，有8名患者的甲状腺滤泡基底膜对相同抗血清呈阳性反应。糖尿病患者和对照患者的所有牙龈和胰腺组织对所有检测的血清蛋白结合均呈阴性。在糖尿病受试者中观察到肾小管和牙龈毛细血管基底膜增厚；然而，糖尿病患者和对照患者在腮腺导管或腺泡基底膜厚度上没有明显差异。
