Murrah V A, Crosson J T, Sauk J J
J Oral Pathol. 1985 Mar;14(3):236-46. doi: 10.1111/j.1600-0714.1985.tb00487.x.
Post-mortem samples of parotid gland were obtained from 15 patients with a history of diabetes mellitus for a minimum of 5 years, and from 15 age- and sex-matched controls. The tissue was studied by direct immunofluorescence for abnormal binding of selected serum proteins, including IgG, IgM, IgA, C3, fibrinogen, polyvalent immunoglobulin and albumin, to acinar and ductal basement membranes of the gland. Thickness of these basement membranes was also assessed using a calibrated magnifier on uniformly enlarged photomicrographs of the tissue which had been stained by the chromotrope silver methenamine method to highlight basement membranes. Results of this investigation revealed parotid gland basement membrane abnormalities in all diabetic subjects as indicated by the binding of IgG, albumin and polyvalent immunoglobulins to ductal and acinar basement membranes. These basement membranes were uniformly negative in control subjects for the binding of all serum proteins tested. Binding of IgA was also noted in 7 of 15 experimental subjects, with 6 of these representing Type I diabetics. Basement membrane measurements revealed no difference in thickness between diabetic and non-diabetic subjects. Variations in parotid diabetic basement membranes evidenced in this study further substantiate the idea that membranopathy in this disease is systemic in nature.
从15名有至少5年糖尿病病史的患者以及15名年龄和性别匹配的对照者身上获取腮腺的尸检样本。通过直接免疫荧光法研究该组织，以检测包括IgG、IgM、IgA、C3、纤维蛋白原、多价免疫球蛋白和白蛋白在内的选定血清蛋白与腮腺腺泡和导管基底膜的异常结合情况。还使用校准放大镜在经变色酸银甲胺法染色以突出基底膜的组织均匀放大显微照片上评估这些基底膜的厚度。这项研究的结果显示，所有糖尿病受试者的腮腺基底膜均存在异常，表现为IgG、白蛋白和多价免疫球蛋白与导管和腺泡基底膜的结合。在对照受试者中，所有检测的血清蛋白结合均呈阴性。在15名实验对象中的7名中也观察到IgA的结合，其中6名是I型糖尿病患者。基底膜测量结果显示糖尿病患者和非糖尿病患者之间的厚度没有差异。本研究中所证明的腮腺糖尿病基底膜的变化进一步证实了这种疾病中的膜病本质上是全身性的这一观点。
