Halbhuber K J, Unger J, Geyer G
Folia Haematol Int Mag Klin Morphol Blutforsch. 1976;103(4):534-40.
Rat erythrocytes previously labelled with 51Cr were treated with 0.1 mM and 0.5 mM dimethyladipic imidate (ADE) pH 9.5, respectively, or with borate buffer pH 9.5 for 15 minutes each. After reinjection into rats of amidinated or nonamidinated erythrocytes their elimination form the streaming blood was followed for 50 days (0.1 mM ADE) or 38 days (0.5 mM ADE). During the 1st day about 10% of amidinated erythrocytes were sequestered. There was no increased initial elimination rate of borate incubated red blood cells detectable. During the following period the elimination rate of erythrocytes amidinated with 0.5 mM ADE was slightly greater than in the case of erythrocytes treated with 0.1 mM ADE. The mean life span of 0.5 mM ADE-erythrocytes was shorter compared with the controls and with 0.1 mM ADE-erythrocytes. Red blood cells previously treated with 0.5 mM ADE are significantly less deformable. Presumably, the stiffness of amidinated erythrocytes causes their increased elimination rate.
预先用51Cr标记的大鼠红细胞分别用0.1 mM和0.5 mM己二酸二甲酯亚胺酯（ADE）（pH 9.5）处理，或用pH 9.5的硼酸盐缓冲液处理，各处理15分钟。将酰胺化或未酰胺化的红细胞重新注入大鼠体内后，追踪其从循环血液中的清除情况，为期50天（0.1 mM ADE）或38天（0.5 mM ADE）。在第1天，约10%的酰胺化红细胞被隔离。未检测到硼酸盐孵育的红细胞初始清除率增加。在接下来的时期，用0.5 mM ADE酰胺化的红细胞的清除率略高于用0.1 mM ADE处理的红细胞。与对照组和0.1 mM ADE处理的红细胞相比，0.5 mM ADE处理的红细胞的平均寿命较短。先前用0.5 mM ADE处理的红细胞的变形能力明显较低。据推测，酰胺化红细胞的僵硬导致其清除率增加。
