Geyer G, Halbhuber K J, Stibenz D, Feuerstein H, Benser A, Meyer H W, Linss W, Claussner A, Brox D
Folia Haematol Int Mag Klin Morphol Blutforsch. 1975;102(3):305-17.
The present studies are concerned with properties of amidinated erythrocytes. The reactions of dimethyladipimidate with proteins in solution and red blood cells, respectively, result in an intermolecular cross-linking. Following an amidination of human serum albumin or human gamma-globulin cross-linked products of increased molecular weight have been demonstrated by polyacrylamide gel and immune electrophoresis. Human erythrocytes previously amidinated intensely, exhibit a restricted motility of membrane particles and cross-linked hemoglobin. Intensely amidinated erythrocytes are resistant against distilled water, and they do no longer agglutinate. The findings presumably indicate an increased permeability of the amidinated red cell membrane. The glycolytic activity was found to be normal in moderately amidinated erythrocytes. In comparison with normal red blood cells, previously moderately amidinated erythrocytes of the rat become sequestered more quickly after re-injection into the vascular system.
目前的研究关注于 amidinated 红细胞的特性。己二酸二甲酯分别与溶液中的蛋白质和红细胞发生反应，会导致分子间交联。在对人血清白蛋白或人γ-球蛋白进行 amidination 后，通过聚丙烯酰胺凝胶和免疫电泳已证明分子量增加的交联产物。先前强烈 amidinated 的人红细胞表现出膜颗粒和交联血红蛋白的运动受限。强烈 amidinated 的红细胞对蒸馏水具有抗性，且不再发生凝集。这些发现大概表明 amidinated 红细胞膜的通透性增加。在适度 amidinated 的红细胞中发现糖酵解活性正常。与正常红细胞相比，先前适度 amidinated 的大鼠红细胞重新注入血管系统后会更快地被隔离。
